NLS-StAx-h combines a nuclear localization sequence with a stapled α-helical domain to promote nuclear targeting and structural stability. Hydrocarbon stapling locks helical conformation, improving resistance to proteolysis. Researchers use it to deliver helix-based motifs into the nucleus and probe transcriptional machinery. Applications include stapled-peptide technology, nuclear delivery, and PPI modulation.
CAT No: R2732
NLS-StAx-h is a synthetic peptide compound designed to incorporate a nuclear localization signal (NLS) motif, making it a valuable tool in molecular and cellular biology research. The peptide's core structure is engineered to facilitate the targeted delivery of molecular cargos to the cell nucleus, leveraging the intrinsic ability of NLS sequences to interact with importin proteins and mediate nuclear import. Its design reflects a focus on studying nucleocytoplasmic transport mechanisms, protein trafficking, and the regulation of nuclear import pathways. As a research-grade peptide, NLS-StAx-h offers a robust platform for probing the dynamics of intracellular localization and the functional consequences of nuclear targeting in various biological contexts.
Protein Delivery Studies: NLS-StAx-h serves as an effective vehicle for investigating protein translocation into the nucleus. By conjugating this peptide to proteins or other biomolecules of interest, researchers can assess the efficiency and specificity of nuclear import mechanisms. This application is particularly valuable in dissecting the role of nuclear localization signals in controlling subcellular distribution, enabling detailed studies of protein function that depend on precise nuclear targeting.
Cellular Imaging and Tracking: The peptide's NLS motif is frequently utilized in live-cell imaging experiments to track the nuclear import of labeled cargos. When linked to fluorescent probes or reporter proteins, NLS-StAx-h allows visualization of nuclear entry in real time, facilitating studies of dynamic trafficking events within living cells. Such imaging applications provide insights into the kinetics of nuclear import, the influence of cellular context, and the effects of experimental perturbations on nucleocytoplasmic transport.
Gene Delivery Research: In gene transfer and transfection studies, NLS-StAx-h is employed to enhance the nuclear delivery of nucleic acids, such as plasmid DNA or oligonucleotides. By attaching the peptide to DNA complexes or nanoparticles, researchers can increase the likelihood of genetic material reaching the nucleus, thereby improving the efficiency of gene expression analysis and functional genomics experiments. This approach is instrumental in optimizing non-viral gene delivery systems for advanced molecular biology workflows.
Mechanistic Studies of Nuclear Import: The peptide provides a defined model for exploring the molecular determinants of nuclear localization signal recognition and transport. By systematically modifying the sequence or structure of NLS-StAx-h, scientists can probe the specificity of importin interactions, map critical residues involved in nuclear import, and elucidate the regulatory factors governing nucleocytoplasmic trafficking. These mechanistic studies contribute to a deeper understanding of cellular compartmentalization and its impact on gene regulation and signal transduction.
Peptide-Protein Interaction Assays: NLS-StAx-h is also valuable in binding studies designed to characterize interactions between NLS-containing peptides and nuclear transport receptors. By employing the peptide in in vitro or cell-based assays, researchers can quantify binding affinities, identify competitive inhibitors, and screen for modulators of nuclear import pathways. Such applications are essential for the discovery of novel regulators of nucleocytoplasmic transport and for validating the functional relevance of NLS motifs in diverse protein families.
1. Cell-based adhesion assays for isolation of snake venom’s integrin antagonists
2. C-Peptide replacement therapy and sensory nerve function in type 1 diabetic neuropathy
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More