3,3',5'-Triiodo-D-thyronine is a non-proteinogenic iodinated thyronine derivative featuring a D-thyronine core bearing three iodine atoms at the 3, 3', and 5' positions on the aromatic rings. The molecule contains a phenolic hydroxyl group and a carboxylic acid functional group as part of the thyronine scaffold, with stereochemistry specified as D in the product name. It is used in chemical biology and analytical method development as a structurally defined iodothyronine analogue for studies of thyroid hormone structure-activity relationships, receptor-binding comparisons, and isotopic or iodine-pattern labeling experiments.
CAT No: CP19502
3,3',5'-Triiodo-D-thyronine is a D-enantiomer thyroid hormone analog bearing three iodine atoms on the thyronine scaffold, making it a structurally defined aromatic amino acid derivative frequently handled as a research-grade standard for thyroid hormone chemistry and analytical workflows. Its heavily iodinated phenolic framework provides strong mass spectrometric detectability and distinctive physicochemical behavior relative to unlabeled or differently iodinated thyronines, which supports its use in method development, reference preparations, and chemical biology studies of thyroid hormone binding and transformation. Researchers typically select this specific stereochemical form when they need an unambiguous chiral and iodine-pattern-defined reagent rather than a generic thyronine mixture.
1. Thyroid Hormone Analytics
3,3',5'-Triiodo-D-thyronine is used as a reference compound for LC-MS and related analytical method development where iodine-pattern and stereochemistry must be controlled to ensure accurate chromatographic separation and mass-based identification of thyronine species. Analytical groups in pharmacokinetics-oriented laboratories, endocrine research facilities, and contract research organizations rely on well-characterized iodinated standards to validate extraction behavior, calibration strategies, and identification criteria for thyroid hormone panels, including studies that compare differently iodinated thyronines and stereoisomeric forms. The compound's multiple iodine atoms provide robust ionization and characteristic isotope-related mass signatures, which simplifies detection and supports reproducible quantitation workflows.
2. Metabolic Transformation Studies
3,3',5'-Triiodo-D-thyronine is applied in metabolic research workflows that examine thyroid hormone biotransformation, including experiments that track conversion between iodinated thyronines and related metabolites in controlled in vitro systems. Biochemistry and chemical biology teams use this defined D-form to distinguish stereochemical outcomes and to interpret transformation patterns without ambiguity from isomeric or differently iodinated standards. In downstream analytical steps, the compound serves both as a starting material for incubation studies and as a structurally matched comparator during metabolite profiling, enabling clearer assignment of parent compound loss and metabolite formation in LC-MS readouts.
3. Chemical Biology Binding Probes
3,3',5'-Triiodo-D-thyronine is employed in chemical biology and protein-binding studies where researchers need a defined, highly iodinated thyronine scaffold to probe interactions with thyroid hormone transport proteins, receptor-associated binding sites, or model binding systems. Molecular biologists and biochemists often use stereochemically specified thyronine analogs to separate binding contributions from iodine substitution pattern and chiral configuration, supporting more rigorous interpretation of binding assays and competition experiments. The aromatic, phenolic-rich structure provides a strong basis for reproducible assay performance in binding and displacement formats, while its distinct identity helps avoid cross-reactivity with closely related thyronines during data interpretation.
4. Research-Grade Reference Material
3,3',5'-Triiodo-D-thyronine is commonly sourced as a high-fidelity reference material for laboratory standard preparation, including preparation of calibration mixes, retention-time/response libraries, and method qualification sets for iodinated thyronines. Analytical chemists and quality-focused research teams value the compound's explicit iodine substitution and D-configuration because these features reduce the risk of misassignment when monitoring complex matrices such as biological extracts or reaction mixtures. In practice, the compound supports consistent benchmarking across instruments and runs, particularly when laboratories need a stable, well-defined comparator for thyronine-specific identification and reporting.
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