4F2 antigen heavy chain (497-510)

4F2 antigen heavy chain (497-510) is a synthetic peptide corresponding to amino acid residues 497 to 510 of the human 4F2 antigen heavy chain, also known as CD98hc or SLC3A2. As a membrane glycoprotein, the 4F2 heavy chain is a critical component of the heterodimeric amino acid transporter complex, playing a key role in cellular amino acid uptake, cell adhesion, and integrin signaling. The 497-510 region of this protein is of particular interest due to its involvement in protein-protein interactions and its accessibility on the extracellular domain, making it a valuable target for biochemical and cell biology research. Investigations into this peptide sequence contribute to a deeper understanding of transporter function, cell surface dynamics, and the molecular mechanisms governing nutrient transport and cellular communication.

Epitope mapping: The 497-510 peptide segment serves as a defined epitope for the generation and characterization of monoclonal and polyclonal antibodies against the 4F2 antigen heavy chain. Researchers utilize this peptide to map antibody binding sites, enabling the development of highly specific immunological reagents for detecting the native protein in various biological samples. Such studies are instrumental in elucidating the spatial organization of the 4F2 heavy chain and in refining antibody-based assays for basic and applied research.

Antibody validation: The synthetic peptide is frequently employed as a positive control in immunoassays, including ELISA, western blotting, and immunoprecipitation, to assess the specificity and sensitivity of antibodies raised against the 4F2 heavy chain. By providing a well-characterized antigenic determinant, it allows researchers to distinguish between specific and nonspecific binding, optimize assay conditions, and ensure the reliability of experimental results involving CD98hc detection.

Protein-protein interaction studies: The 4F2 antigen heavy chain (497-510) peptide is used in binding assays to investigate interactions with partner proteins, ligands, or small molecules. Its defined sequence enables precise probing of the extracellular interface of the 4F2 heavy chain, facilitating studies on how this region mediates heterodimer formation with the light chain subunit and interacts with integrins or other cell surface receptors. Such applications advance the understanding of transporter assembly and cell adhesion processes.

Cell surface labeling and imaging: By serving as an accessible extracellular epitope, the 497-510 peptide is valuable for developing fluorescently labeled probes or affinity tags for live-cell imaging and flow cytometry. Researchers can use labeled derivatives to selectively stain cells expressing the 4F2/CD98 complex, enabling visualization and quantification of transporter distribution, surface expression dynamics, and cell population heterogeneity in real time.

Peptide competition assays: The synthetic peptide is applied in competitive binding experiments to assess the functional relevance of the 497-510 region in ligand recognition or antibody binding. By competing with the native protein for interaction partners, it helps delineate the contribution of this specific segment to overall transporter function and signaling. These assays provide mechanistic insights into the regulation of amino acid transport and the modulation of cell surface receptor activity.

1. A possible role of tachykinin-related peptide on an immune system activity of mealworm beetle, Tenebrio molitor L

2. C-peptide and its C-terminal fragments improve erythrocyte deformability in type 1 diabetes patients

3. Genetic, cellular, and structural characterization of the membrane potential-dependent cell-penetrating peptide translocation pore

4. Store-operated Ca2+ entry sustains the fertilization Ca2+ signal in pig eggs

5. Autoinhibition and phosphorylation-induced activation of phospholipase C-γ isozymes