Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.
CAT No: R1308
CAS No:171783-05-4
Synonyms/Alias:Crosstide;171783-05-4;Crosstide (trifluoroacetate salt);MFCD03787963;DTXSID60432141;AS-85941;Glycyl-L-arginyl-L-prolyl-L-arginyl-L-threonyl-L-seryl-L-seryl-L-phenylalanyl-L-alanyl-L-alpha-glutamylglycine;A11762;Crosstide, >=97% (HPLC), lyophilized powder;(4S)-4-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S,3R)-2-[(2S)-2-{[(2S)-1-[(2S)-2-(2-AMINOACETAMIDO)-5-CARBAMIMIDAMIDOPENTANOYL]PYRROLIDIN-2-YL]FORMAMIDO}-5-CARBAMIMIDAMIDOPENTANAMIDO]-3-HYDROXYBUTANAMIDO]-3-HYDROXYPROPANAMIDO]-3-HYDROXYPROPANAMIDO]-3-PHENYLPROPANAMIDO]PROPANAMIDO]-4-(CARBOXYMETHYLCARBAMOYL)BUTANOIC ACID;
Crosstide is a synthetic peptide substrate recognized for its utility in kinase research, particularly as a tool for probing the activity of specific serine/threonine kinases such as Akt/PKB and related AGC family kinases. Structurally, Crosstide is designed to mimic natural phosphorylation motifs, enabling it to serve as a reliable and sensitive reporter in biochemical assays. Its sequence incorporates a critical phosphorylation site, making it highly applicable for in vitro kinase activity measurements, substrate specificity studies, and the development of high-throughput screening platforms. The compound's defined peptide structure and compatibility with various detection methods have established it as a valuable asset in the study of signal transduction pathways and enzyme kinetics.
Kinase activity assays: Crosstide is widely employed as a substrate in kinase assays to quantitatively monitor the enzymatic activity of serine/threonine kinases, especially Akt/PKB. By providing a specific phosphorylation site, it enables precise assessment of kinase function through incorporation of radiolabeled phosphate or detection via phospho-specific antibodies. Researchers utilize this peptide to evaluate the catalytic efficiency of kinases, compare isoform selectivity, and investigate the impact of small molecule inhibitors or activators on phosphorylation events. Such assays are fundamental to elucidating cellular signaling mechanisms and validating potential drug targets.
Signal transduction research: The peptide's sequence closely resembles consensus motifs found in endogenous kinase substrates, making it a powerful tool for dissecting intracellular signaling cascades. Scientists employ Crosstide to map kinase-substrate relationships, profile pathway dynamics, and characterize regulatory feedback within complex networks. Its application extends to studies of growth factor signaling, metabolic regulation, and stress response pathways, where accurate measurement of phosphorylation events is critical for understanding cellular decision-making processes.
High-throughput screening: In pharmaceutical and academic research settings, Crosstide serves as a robust substrate in high-throughput screening (HTS) formats aimed at identifying novel modulators of kinase activity. Its compatibility with fluorescence-based, luminescent, and radiometric detection systems facilitates rapid and reproducible quantification of phosphorylation, enabling efficient screening of compound libraries. The peptide's defined sequence and performance consistency help streamline assay development and support the discovery of new chemical entities with potential therapeutic relevance.
Enzyme kinetics and substrate profiling: The defined nature of Crosstide allows for detailed kinetic characterization of kinase-substrate interactions. By systematically varying substrate concentration and monitoring reaction rates, researchers can determine key enzymatic parameters such as Km and Vmax, as well as investigate substrate specificity and cross-reactivity among kinase family members. These studies provide mechanistic insight into enzyme function, inform inhibitor design, and contribute to the rational development of targeted research tools.
Analytical method development: Beyond its role in functional assays, Crosstide is utilized in the optimization and validation of analytical techniques for phosphopeptide detection and quantification. Its predictable phosphorylation behavior makes it an ideal standard for method calibration in mass spectrometry and immunodetection platforms. By serving as a reference substrate, it assists in benchmarking assay sensitivity, linearity, and reproducibility, thereby enhancing the reliability of quantitative phosphoproteomics and related analytical workflows.
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