CTAG is a peptide associated with cancer-testis antigen families, containing motifs relevant to epitope presentation and protein interaction. Researchers use it to explore MHC-loading behavior, structural plasticity, and solvent-dependent folding. Its well-defined nature suits immunological and biochemical testing.
CAT No: ta-432
CTAG is a synthetic peptide compound composed of the amino acid sequence cysteine-threonine-alanine-glycine. As a tetrapeptide, it is valued for its defined structure and versatile biochemical properties, making it a useful tool in peptide research and molecular biology. The sequence of CTAG allows for targeted investigations into peptide-protein interactions, enzymatic specificity, and structure-activity relationships, offering researchers a reliable model for studying fundamental aspects of peptide function. Its small size and the presence of a reactive cysteine residue further enhance its utility in conjugation strategies and bioconjugate chemistry, supporting a range of experimental applications in life sciences.
Peptide synthesis calibration: CTAG serves as a well-characterized standard for calibrating peptide synthesis protocols, particularly in solid-phase peptide synthesis (SPPS) workflows. Its defined sequence and manageable length make it ideal for optimizing coupling efficiency, resin loading, and cleavage conditions. Researchers routinely employ this tetrapeptide as a benchmark to assess the fidelity of synthetic methodologies, troubleshoot synthesis issues, and validate purification strategies, thereby ensuring the reproducibility and accuracy of custom peptide production.
Enzyme substrate studies: The CTAG peptide is frequently utilized as a model substrate for examining the specificity and kinetics of proteolytic enzymes, such as cysteine proteases and serine proteases. Its sequence, especially the N-terminal cysteine, provides a suitable motif for exploring enzyme recognition and cleavage preferences. By monitoring proteolytic processing of CTAG, scientists can gain insights into enzyme mechanism, substrate selectivity, and inhibitor screening, contributing to the broader understanding of protease biology and regulation.
Peptide-protein interaction assays: Researchers leverage CTAG in binding studies to elucidate the molecular determinants of peptide-protein interactions. The tetrapeptide can be labeled or immobilized via its cysteine residue, enabling its use in affinity chromatography, surface plasmon resonance (SPR), or fluorescence-based assays. These applications facilitate the identification and characterization of binding partners, mapping of interaction domains, and quantification of binding affinities, which are essential steps in the discovery of novel biomolecular interactions.
Conjugation and labeling strategies: The presence of a free thiol group on the cysteine residue in CTAG allows for site-specific conjugation to a variety of functional moieties, such as fluorophores, biotin, or carrier proteins. This functionalization capability makes it a valuable scaffold for preparing labeled peptides, constructing peptide-based probes, and developing targeted delivery systems. Such modifications are instrumental in imaging studies, assay development, and the creation of multifunctional peptide conjugates for advanced research applications.
Structural and biophysical analysis: CTAG is often employed as a model system in studies aimed at understanding peptide folding, conformational dynamics, and aggregation behavior. Its short, defined sequence permits detailed analysis using techniques such as nuclear magnetic resonance (NMR) spectroscopy, circular dichroism (CD), or mass spectrometry. Data obtained from these studies inform the design of more complex peptides, contribute to computational modeling efforts, and advance knowledge of the principles governing peptide structure and stability in various environments.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More