3,5-Diiodo-DL-thyronine contains the thyronine scaffold bearing two iodine substituents at the 3- and 5-positions on the aromatic rings, classifying it as a halogenated, non-proteinogenic amino acid derivative rather than a standard proteinogenic amino acid. The molecule features an amino group and a carboxyl group consistent with an amino acid framework, and the "DL" designation indicates a racemic mixture of stereoisomers at the chiral center(s) associated with the thyronine structure. In research settings, this iodinated thyronine analogue is used as a chemically defined substrate or reference for studies involving thyroid-hormone analogs, iodine substitution patterns, and analytical method development for labeled or halogenated biomolecule detection.
CAT No: CP18303
3,5-Diiodo-DL-thyronine is an iodinated thyronine derivative used as a chemically defined reference material in analytical and chemical biology workflows. The DL stereochemical designation indicates a racemic mixture, and the two iodine substituents make the compound particularly relevant for mass spectrometry-based quantification and iodine-containing tracer studies where isotopic or elemental iodine content is a key analytical variable. In practice, it is handled as a stable, non-radioactive iodinated building block for method development, calibration, and downstream synthesis of thyronine-related analogs.
1. Iodinated Standards And Calibration
3,5-Diiodo-DL-thyronine is routinely used to prepare analytical standards for LC-MS workflows targeting iodinated thyroid-mimetic chemotypes. Researchers in bioanalytical chemistry and environmental/chemical analysis laboratories rely on this compound to validate retention behavior, ionization response, and mass-based identification for iodine-rich small molecules. The presence of two iodine atoms provides a distinctive mass signature that supports robust qualifier/quantifier selection during method development and routine runs, including studies where separation of closely related thyronine derivatives is required.
2. Thyronine Analog Intermediate Synthesis
3,5-Diiodo-DL-thyronine serves as a practical starting material for pharmaceutical intermediate development and medicinal chemistry programs focused on thyronine analogs and iodinated aromatic scaffolds. Organic synthesis teams use it to construct or diversify iodine-substituted phenyl/thyronine frameworks, enabling access to substituted derivatives used in SAR exploration and chemical library generation. The racemic DL form is often selected for intermediate supply when downstream steps will establish or separate stereochemistry later, or when the immediate goal is to access a defined iodinated scaffold for further functionalization.
3. Mass Spectrometry Tracing Studies
3,5-Diiodo-DL-thyronine is applied in chemical biology and analytical research where iodine content and thyronine-like structure are monitored as part of tracer or distribution experiments. Analytical groups use it as a non-radioactive reference compound to support calibration and to benchmark extraction efficiency, matrix effects, and recovery in complex sample types such as biological extracts or formulation-like matrices. Its iodinated substitution pattern makes it useful for distinguishing target-related signals from background in MS-based workflows, particularly when multiple iodinated species are expected.
4. Iodinated Reagent For Method Verification
3,5-Diiodo-DL-thyronine is also used as a verification reagent in quality control of analytical reagents and sample preparation procedures. Laboratories performing routine quantification of iodinated small molecules incorporate it to check consistency across batches, including stability of prepared standard solutions and reproducibility of derivatization or cleanup steps where applicable. Because the compound is structurally well-defined and contains two iodine atoms, it provides a reliable structural reference point for confirming that the analytical pipeline is responding correctly to iodinated thyronine motifs.
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