Fmoc-Ala-Glu-Asn-Lys-NH2 combines a protecting group with a functional tetrapeptide used in synthetic route optimization. Acidic, neutral, and basic residues create a well-balanced system for conformational assessment. The C-terminal amide supports stability during coupling and deprotection steps. Researchers apply it to resin-bound synthesis testing, sequence expansion, and bioconjugation strategy evaluation.
CAT No: R2327
CAS No:220701-06-4
Synonyms/Alias:Fmoc-Ala-Glu-Asn-Lys-NH2;220701-06-4;AKOS040756234;MS-31095;HY-114174;CS-0078435;G17225;(4S)-5-[[(2S)-4-amino-1-[[(2S)-1,6-diamino-1-oxohexan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-4-[[(2S)-2-(9H-fluoren-9-ylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoic acid;
Fmoc-Ala-Glu-Asn-Lys-NH2 is a synthetic tetrapeptide featuring the Fmoc (9-fluorenylmethyloxycarbonyl) protective group at the N-terminus and an amidated C-terminus. This protected peptide is specifically designed for use in solid-phase peptide synthesis (SPPS), providing researchers with a versatile building block for the assembly of longer peptide chains or for the incorporation of specific sequence motifs into custom peptides. The presence of alanine, glutamic acid, asparagine, and lysine within its sequence imparts a balanced combination of hydrophobic, acidic, polar, and basic side chains, making it suitable for applications that require structural diversity and functional group availability. The Fmoc protection ensures compatibility with standard Fmoc/tBu SPPS protocols, while the C-terminal amide enhances the mimicry of native peptide backbones, supporting bioactivity and stability in downstream applications.
Peptide Synthesis: Fmoc-Ala-Glu-Asn-Lys-NH2 serves as a valuable intermediate in the stepwise synthesis of complex peptides. Its pre-assembled tetrapeptide structure streamlines the elongation process, reducing the number of coupling cycles and minimizing the risk of sequence errors or incomplete reactions. By incorporating this protected peptide into SPPS workflows, researchers can efficiently construct larger peptides or protein fragments with precise sequence fidelity. The use of Fmoc chemistry facilitates easy deprotection under mild conditions, preserving sensitive side chains and maintaining the integrity of the growing peptide chain.
Structure-Activity Relationship Studies: In the context of structure-activity relationship (SAR) investigations, this tetrapeptide offers a modular platform for evaluating the effects of specific amino acid arrangements on biological function. Researchers can utilize Fmoc-Ala-Glu-Asn-Lys-NH2 to systematically substitute or modify residues within larger peptide libraries, enabling the identification of key sequence motifs responsible for target binding or functional activity. The inclusion of both charged and neutral residues within this sequence allows for the exploration of electrostatic and hydrophobic interactions in peptide-target recognition.
Protein-Protein Interaction Research: The sequence of Ala-Glu-Asn-Lys is representative of motifs found in various protein-protein interaction domains. By incorporating this peptide into binding assays or pull-down experiments, scientists can probe the molecular determinants of protein association, map interaction interfaces, or design competitive inhibitors for functional studies. The amidated C-terminus enhances the peptide's resemblance to natural protein segments, improving the relevance of experimental results in mimicking physiological conditions.
Epitope Mapping and Antibody Production: Fmoc-Ala-Glu-Asn-Lys-NH2 can be utilized in epitope mapping experiments to identify antibody binding sites within larger protein antigens. Its defined sequence and modifiable termini enable the generation of peptide panels for screening with monoclonal or polyclonal antibodies. This approach supports the elucidation of antigenic determinants, guiding the development of diagnostic reagents or the optimization of immunogens for antibody production. The synthetic accessibility of the peptide ensures consistent quality and reproducibility across experimental batches.
Peptide-Based Material Science: Beyond biological research, this tetrapeptide finds utility in the design of peptide-based materials and nanostructures. Its sequence diversity and compatibility with chemical modifications allow researchers to tailor the assembly properties, surface functionalities, or responsiveness of peptide-derived hydrogels, films, or nanoparticles. By leveraging the unique side chain characteristics of alanine, glutamic acid, asparagine, and lysine, scientists can engineer materials with tunable charge, hydrophilicity, or bioactivity for applications in biosensing, drug delivery, or tissue engineering. The Fmoc group also facilitates further functionalization or conjugation strategies, expanding the scope of material innovation.
1. Myotropic activity of allatostatins in tenebrionid beetles
2. Cationic cell-penetrating peptides are potent furin inhibitors
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More