G-protein coupled receptor 143
G-protein coupled receptor 143 (126-134) is a synthetic peptide fragment corresponding to amino acids 126 through 134 of the human G-protein coupled receptor 143 (GPCR143), also known as ocular albinism type 1 protein. As a segment of a class A GPCR, this peptide is of significant interest in the study of receptor structure, ligand interaction, and signal transduction processes. Its defined sequence enables precise modeling of receptor domains, facilitating biochemical investigations into the molecular mechanisms underlying GPCR function, trafficking, and protein-protein interactions. Researchers leverage such peptide fragments to dissect the role of specific receptor regions in cellular signaling and to develop targeted analytical assays in receptor biology.
Receptor-ligand interaction studies: The peptide fragment representing residues 126-134 of GPCR143 serves as a valuable tool for elucidating the molecular determinants of ligand binding within the receptor. By incorporating this peptide into binding assays or structural analyses, researchers can investigate the contribution of the 126-134 region to ligand recognition, specificity, and affinity. Such studies are critical for mapping binding epitopes, identifying potential allosteric sites, and informing the rational design of receptor-targeted probes or modulators.
Antibody generation and epitope mapping: The defined sequence of this peptide makes it an ideal antigen for the production of sequence-specific antibodies against GPCR143. Immunization protocols utilizing the peptide can yield polyclonal or monoclonal antibodies that recognize the corresponding region of the native receptor, enabling selective detection in immunoblotting, immunoprecipitation, or immunohistochemistry applications. Furthermore, the peptide can be employed in epitope mapping experiments to characterize antibody binding sites, assess cross-reactivity, and refine immunoassay specificity.
Signal transduction research: As a functional motif of a G-protein coupled receptor, this peptide fragment can be utilized in studies aimed at dissecting intracellular signaling pathways. Synthetic peptides corresponding to receptor domains are frequently used to probe G-protein coupling, receptor desensitization, or downstream effector activation. By introducing the 126-134 fragment into cellular or in vitro systems, investigators can analyze its influence on signaling cascades, providing mechanistic insights into the dynamics of GPCR-mediated responses.
Protein-protein interaction analysis: The 126-134 region of GPCR143 may participate in interactions with intracellular scaffolding proteins, regulatory molecules, or other components of the signaling machinery. Synthetic peptides corresponding to this region are instrumental in pull-down assays, surface plasmon resonance studies, or crosslinking experiments designed to identify and characterize binding partners. These approaches help clarify the structural and functional context of receptor complexes, advancing the understanding of GPCR organization and regulation.
Peptide-based assay development: The availability of a well-defined GPCR143-derived peptide supports the development of sensitive and selective biochemical assays. Such peptides are commonly used as standards or substrates in quantitative analyses, including mass spectrometry, enzyme-linked immunosorbent assays (ELISAs), or fluorescence-based screening platforms. Incorporation of the 126-134 fragment into assay systems enables precise quantitation, facilitates method validation, and enhances the reliability of receptor-focused research workflows.
1. Emerging applications of nanotechnology for diagnosis and therapy of disease: a review
4. Adipose tissue is a key organ for the beneficial effects of GLP-2 metabolic function
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More