GluN1(359-378) corresponds to a defined fragment of the NMDA receptor GluN1 subunit used to probe protein-protein interactions. The peptide contains acidic, polar, and hydrophobic residues that shape epitope structure. Researchers employ it in binding assays, antibody mapping, and structural modeling. Applications include synaptic-signaling research, receptor-domain characterization, and motif-function analysis.
CAT No: R2867
GluN1(359-378) is a synthetic peptide fragment corresponding to amino acid residues 359 to 378 of the GluN1 subunit, a critical component of the N-methyl-D-aspartate (NMDA) receptor complex. As a highly conserved region within the NMDA receptor, GluN1(359-378) is of significant interest in neurobiological research, particularly in studies focused on synaptic transmission, receptor assembly, and signal modulation. The peptide's sequence encompasses a functionally relevant domain involved in receptor activity and protein-protein interactions, making it a valuable molecular tool for elucidating the structural and functional aspects of NMDA receptor physiology.
Receptor Structure-Function Analysis: Researchers utilize GluN1(359-378) to investigate the structural determinants of NMDA receptor assembly and gating. By employing this peptide in binding assays, mutagenesis studies, or as a competitor in functional assays, scientists can dissect the contribution of this specific region to receptor conformation, channel opening, and subunit interface formation. Such studies advance the understanding of how sequence-specific motifs within the GluN1 subunit influence receptor architecture and bioactivity.
Protein-Protein Interaction Mapping: The peptide serves as a useful probe for identifying and characterizing binding partners that interact with the intracellular domains of the NMDA receptor. Pull-down assays, co-immunoprecipitation, and surface plasmon resonance experiments often employ GluN1(359-378) to map interaction sites with scaffolding proteins, kinases, or regulatory factors. These investigations reveal critical insights into the molecular networks governing synaptic signaling and receptor modulation.
Antibody Generation and Epitope Mapping: GluN1(359-378) is frequently used as an immunogen for the production of sequence-specific antibodies targeting the NMDA receptor. Such antibodies are essential tools for immunohistochemistry, western blotting, and immunoprecipitation, enabling precise localization and quantification of the GluN1 subunit in complex biological samples. Additionally, the peptide supports epitope mapping efforts, facilitating the validation of antibody specificity and the identification of immunoreactive domains.
Phosphorylation and Post-Translational Modification Studies: The defined amino acid sequence of GluN1(359-378) allows for controlled in vitro studies of post-translational modifications, such as phosphorylation by specific kinases. By serving as a substrate in kinase assays or mass spectrometry analyses, the peptide enables detailed investigation into the regulatory mechanisms that modulate NMDA receptor function through modification of intracellular domains. These studies are pivotal for understanding signal integration and plasticity in neuronal systems.
Peptide-Based Assay Development: The well-characterized nature of GluN1(359-378) makes it an ideal standard or reference in the development of biochemical and biophysical assays targeting NMDA receptor components. It is used in ELISA, competitive binding assays, and biosensor platforms to calibrate detection systems or to quantify interactions involving the GluN1 subunit. Such applications are integral to advancing high-throughput screening and quantitative analysis in neuropharmacological research and drug discovery.
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