Growth arrest-specific protein 7
Growth arrest-specific protein 7 (281-290) is a synthetic peptide fragment derived from the C-terminal region of the human GAS7 protein, a member of the growth arrest-specific gene family. Characterized by its involvement in neuronal development and cytoskeletal regulation, this peptide segment represents a valuable tool for researchers investigating protein-protein interactions, post-translational modifications, and signaling pathways associated with cellular differentiation and growth arrest. Its well-defined sequence enables precise studies into the structural and functional domains of GAS7, thereby supporting advanced research in neurobiology, cell signaling, and peptide-based assay development.
Peptide mapping: The 281-290 fragment of GAS7 serves as a critical probe for mapping functional domains within the parent protein. By isolating this specific sequence, researchers can examine its role in mediating interactions with cytoskeletal elements such as actin and tubulin, which are essential for neurite outgrowth and morphological differentiation. Utilizing the peptide in in vitro binding assays or pull-down experiments enables the dissection of binding affinities and specificities, thereby elucidating the molecular mechanisms underlying GAS7-mediated cellular processes.
Antibody production: The defined sequence of the GAS7 (281-290) peptide offers a highly immunogenic epitope for generating sequence-specific antibodies. Such antibodies are instrumental in selectively detecting GAS7 or its post-translationally modified forms in complex biological samples. Application of these antibodies in Western blotting, immunoprecipitation, or immunofluorescence assays facilitates the study of GAS7 expression patterns, subcellular localization, and protein turnover in neuronal and non-neuronal contexts.
Signal transduction studies: The peptide fragment provides a focused tool for investigating the signaling pathways regulated by GAS7, particularly those involved in growth arrest and neuronal differentiation. Researchers can employ the peptide in cell-based assays to monitor its influence on downstream effectors, phosphorylation events, or protein recruitment. This approach aids in delineating the contribution of the 281-290 region to the modulation of key signaling cascades, thereby advancing understanding of GAS7's regulatory functions.
Peptide-protein interaction assays: As a structurally defined segment, the GAS7 (281-290) peptide is suitable for use in surface plasmon resonance, isothermal titration calorimetry, or fluorescence polarization assays to quantitatively assess its binding interactions with partner proteins. These studies are critical for determining the affinity and kinetics of GAS7-mediated complexes, which has implications for understanding the assembly and regulation of multi-protein structures in the cytoskeleton and beyond.
Structure-activity relationship (SAR) analysis: The availability of the 281-290 peptide enables systematic SAR studies to identify key residues responsible for biological activity or molecular recognition. Researchers can introduce targeted modifications, such as alanine scanning or incorporation of non-natural amino acids, to evaluate the contribution of individual side chains to binding or functional outcomes. Such investigations provide mechanistic insights into the structure-function relationships within GAS7 and inform the rational design of peptide mimetics or inhibitors for research applications.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More