H-[15N]Gly-OH

H-[15N]Gly-OH is an isotopically labeled glycine in which the backbone nitrogen is enriched with 15N, retaining the simple amino acid structure of NH2-CH2-CO2H while providing a defined chiral-independent labeling site. The molecule contains a primary amino group and a carboxylic acid, enabling standard amino acid chemistry for salt formation, activation, and peptide coupling under conditions compatible with glycine building blocks. The 15N label produces a distinct isotopic signature that can be tracked by isotope-sensitive spectroscopic and mass spectrometric methods without altering the fundamental reactivity of the glycine scaffold. As a chemically minimal amino acid, H-[15N]Gly-OH functions as a clean biochemical research intermediate for downstream incorporation into peptides, proteins, and analytical reference materials.

1. Isotope Tracing Studies

H-[15N]Gly-OH supports isotope tracing in biochemical research and analytical chemistry because the 15N label resides directly on the amino nitrogen of glycine. The amino and carboxylic acid functionalities allow conversion into activated glycine derivatives for incorporation into peptide backbones while preserving the isotopic marker for downstream detection. Isotope-sensitive NMR, MS, and IR approaches can exploit the labeled nitrogen to monitor incorporation, metabolic turnover in enzymatic systems, or stability of labeled peptide segments. The resulting labeled biomolecules serve as quantitative reporters for amino acid utilization and backbone remodeling pathways in mechanistic studies.

2. Peptide NMR Labeling

H-[15N]Gly-OH is suitable for peptide NMR labeling workflows where selective enrichment of the glycine amide nitrogen improves spectral assignment and resonance tracking. The small, non-chiral glycine side chain minimizes conformational perturbation, while the carboxylic acid and amino group enable peptide building block preparation through standard coupling-compatible transformations. Incorporation into peptide sequences can be designed to place the 15N label at specific backbone positions, supporting structural characterization, conformational analysis, and sequential assignment strategies for short peptides and peptide fragments. Labeled peptide products derived from H-[15N]Gly-OH can be used as reference materials for method development in structural biology and peptide analytics.

3. Protein Engineering Workflows

H-[15N]Gly-OH can be applied to protein engineering and chemical protein modification strategies that require site-specific nitrogen isotopic labeling at glycine positions. The amino acid functionality enables incorporation into peptide ligation segments, protease substrates, or chemically synthesized protein fragments where glycine residues are introduced as defined building units. The 15N label can then be followed in spectroscopic readouts to evaluate local environment changes, proteolysis patterns, or backbone dynamics in engineered constructs. The labeled intermediates and final peptide/protein materials can function as research-grade tools for mapping structural features tied to glycine-containing motifs.

4. Mass Spectrometry Standards

H-[15N]Gly-OH is appropriate for analytical research and method validation where isotopically labeled amino acids improve calibration, internal standards, and identification workflows. The labeled nitrogen provides a predictable mass shift relative to unlabeled glycine, while the intact amino acid core supports derivatization into common analytical forms such as salts or coupling-compatible derivatives. The compound can be used to generate labeled peptide standards that mimic analyte behavior during LC-MS/MS, improving confidence in quantitation and structural confirmation. Downstream labeled reference materials prepared from H-[15N]Gly-OH can support routine quality control of peptide-based assays and isotope-aware analytical pipelines.

5. Industrial Fine Chemical Intermediates

H-[15N]Gly-OH serves as a manufacturing-oriented intermediate for producing isotopically labeled amino acid building blocks and labeled peptide reagents used in biochemical research supply chains. The molecule's functional group set, consisting of a primary amino group and a carboxylic acid, supports controlled conversion into activated forms compatible with peptide synthesis and downstream incorporation into labeled scaffolds. Process chemistry routes can leverage glycine's minimal steric profile to streamline handling and integration into labeling strategies for peptide fragments and analytical standards. The resulting labeled materials support industrial production of isotope-tagged reagents for analytical method development, reagent qualification, and research-grade peptide manufacturing.

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