H-Val-Pro-Pro-OH (TFA), a milk-derived proline peptides derivative, is an inhibitor of Angiotensin I converting enzyme (ACE), with an IC50 of 9 μM.
CAT No: R1438
H-Val-Pro-Pro-OH TFA is a synthetic tripeptide composed of valine, proline, and proline residues, supplied as its trifluoroacetic acid (TFA) salt. As a chemically defined peptide, it serves as a valuable tool in biochemical and molecular research, particularly in studies exploring peptide structure-activity relationships, enzymatic processing, and receptor interactions. The sequence arrangement of valine followed by two prolines imparts unique conformational properties, making this tripeptide relevant for investigations into proline-rich motifs and their biological significance. Its defined composition and manageable size also facilitate its use as a model peptide in a range of experimental settings.
Peptide structure-function analysis: H-Val-Pro-Pro-OH TFA is frequently employed in research focused on elucidating the structural determinants of peptide function, especially those involving proline-rich sequences. The presence of consecutive proline residues introduces conformational rigidity, influencing the overall three-dimensional structure of the peptide. By incorporating this tripeptide into experimental systems, researchers can systematically assess how proline-induced kinks and turns affect peptide binding, stability, and recognition by proteins such as peptidases or proline-specific binding partners.
Enzymatic substrate studies: The tripeptide serves as a model substrate for investigating the specificity and catalytic mechanisms of proteolytic enzymes, particularly those that recognize or cleave at proline-containing sites. Its defined sequence allows for precise kinetic and mechanistic studies, enabling researchers to dissect enzyme-substrate interactions, determine cleavage preferences, and characterize the influence of adjacent amino acids on enzymatic activity. Such insights are critical for understanding proteolytic processing in physiological and pathological contexts.
Peptide synthesis and method validation: In peptide chemistry, H-Val-Pro-Pro-OH TFA is utilized as a reference standard or intermediate for optimizing solid-phase peptide synthesis protocols. The unique challenges associated with coupling and deprotection of proline-rich sequences make this tripeptide a useful benchmark for evaluating coupling efficiency, resin compatibility, and purification strategies. Its application in method development contributes to improved reliability and reproducibility in the synthesis of more complex peptides.
Receptor binding and interaction assays: The defined structure of this tripeptide enables its use in binding studies with receptors, antibodies, or other peptide-interacting proteins. Researchers can employ it in in vitro assays to probe the specificity and affinity of interactions involving proline-rich motifs, which are often implicated in signal transduction, cellular adhesion, or immune recognition. Such studies support the identification of critical binding determinants and the development of peptide-based probes or inhibitors.
Analytical calibration and instrument testing: Due to its well-characterized molecular properties, H-Val-Pro-Pro-OH TFA can be applied as a calibration standard in analytical chemistry, particularly in mass spectrometry and chromatography. Its use aids in instrument performance verification, method calibration, and troubleshooting, ensuring accurate detection and quantification of peptides in complex mixtures. Employing defined peptides for these purposes enhances data quality and supports rigorous analytical workflows in both research and quality control environments.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More