Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).
CAT No: R1466
CAS No:65189-71-1
Synonyms/Alias:Kemptide;65189-71-1;H-Leu-Arg-Arg-Ala-Ser-Leu-Gly-OH;MFCD00076710;CHEMBL152998;SCHEMBL7741846;WIGDGIGALMYEBW-LLINQDLYSA-N;Glycine,L-leucyl-L-arginyl-L-arginyl-L-alanyl-L-seryl-L-leucyl-;HY-P0248;AKOS040763980;NCGC00167224-01;DB-277013;A12727;Kemptide acetate (H-DL-Leu-DL-Arg-DL-Arg-DL-Ala-DL-Ser-DL-Leu-Gly-OH);L-LEUCYL-L-ARGINYL-L-ARGINYL-L-ALANYL-L-SERYL-L-LEUCYL-GLYCINE;(5S,8S,11S,14S,17S,20S)-20-amino-14,17-bis(3-guanidinopropyl)-8-(hydroxymethyl)-5-isobutyl-11,22-dimethyl-4,7,10,13,16,19-hexaoxo-3,6,9,12,15,18-hexaazatricosan-1-oic acid;[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-amino-4-methylpentanamido]-5-carbamimidamidopentanamido]-5-carbamimidamidopentanamido]propanamido]-3-hydroxypropanamido]-4-methylpentanamido]acetic acid;2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylpentanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]propanoyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]acetic acid;
Kemptide is a synthetic peptide substrate widely recognized for its pivotal role in protein kinase research and signal transduction studies. Structurally, it is a heptapeptide that closely mimics consensus phosphorylation sequences, making it an invaluable tool for investigating kinase activity, substrate specificity, and phosphorylation-dependent cellular processes. Its well-defined sequence and reactivity have established it as a gold standard substrate in biochemical assays, particularly those focused on cAMP-dependent protein kinase (PKA) and related serine/threonine kinases. The versatility and specificity of Kemptide enable researchers to dissect complex phosphorylation events and elucidate the molecular mechanisms underlying cellular regulation.
Kinase activity assays: Kemptide is extensively employed as a model substrate in in vitro kinase assays to quantitatively measure the catalytic activity of cAMP-dependent protein kinase (PKA) and other serine/threonine kinases. Its defined amino acid sequence provides a reliable target for phosphorylation, allowing for precise monitoring of enzyme kinetics, substrate turnover, and inhibitor screening. This application is fundamental in characterizing kinase function, evaluating the potency of kinase modulators, and supporting drug discovery efforts targeting phosphorylation pathways.
Signal transduction research: As a substrate that closely resembles physiological phosphorylation sites, Kemptide enables detailed investigation of signaling cascades involving PKA and related kinases. By serving as a reporter in phosphorylation assays, it facilitates the study of intracellular signaling dynamics, feedback regulation, and cross-talk between signaling networks. Researchers leverage its specificity to unravel the roles of kinases in cellular responses to hormonal, metabolic, or environmental stimuli.
Enzyme specificity profiling: The use of Kemptide in comparative studies allows for the assessment of substrate preferences among different kinases. By analyzing the efficiency and selectivity with which various kinases phosphorylate this peptide, scientists can map substrate recognition motifs and identify structural determinants of enzyme-substrate interactions. Such profiling is crucial for understanding kinase-substrate relationships and for designing more selective kinase inhibitors or engineered proteins.
High-throughput screening: The robust and reproducible phosphorylation of Kemptide makes it well-suited for high-throughput assay platforms used in pharmaceutical and academic research. Automated screening protocols utilize this peptide to rapidly evaluate large compound libraries for kinase modulatory activity, facilitating the identification of potential therapeutic leads or research probes. Its compatibility with radiometric, colorimetric, and fluorescence-based detection formats further enhances its utility in diverse screening workflows.
Peptide-based assay development: The defined sequence and phosphorylation responsiveness of Kemptide support its use in the optimization and validation of peptide-based kinase assays. Researchers employ it to calibrate assay conditions, benchmark new detection technologies, and develop standardized protocols for measuring kinase activity. Its performance as a reference substrate ensures consistency and comparability across experimental systems, making it a cornerstone in assay development for biochemical and pharmacological research.
2. Implications of ligand-receptor binding kinetics on GLP-1R signalling
3. The spatiotemporal control of signalling and trafficking of the GLP-1R
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