KIAA0809 protein, partial (881-890)

KIAA0809 protein, partial (881-890) represents a synthetic peptide fragment derived from the C-terminal region of the human KIAA0809 protein. As a segment corresponding to amino acid residues 881 through 890, this peptide encompasses a sequence of interest for researchers investigating the structure, function, and biological interactions of the parent protein. KIAA0809 itself is a member of a family of large, uncharacterized proteins encoded by the KIAA gene collection, which has been implicated in diverse cellular processes based on emerging high-throughput proteomics and transcriptomics studies. The partial peptide fragment offers a targeted approach for dissecting the specific roles of defined regions within the full-length protein, facilitating a range of experimental strategies in molecular biology, protein chemistry, and cell signaling research.

Epitope mapping: The partial (881-890) peptide is particularly valuable for epitope mapping applications, enabling researchers to identify and characterize antibody binding sites within the C-terminal domain of the KIAA0809 protein. By serving as a defined antigenic determinant, the peptide can be used in immunoassays such as ELISA or western blotting to confirm antibody specificity or to develop custom antibodies that recognize the native or denatured forms of the full-length protein. This approach supports the generation of highly selective immunoreagents for downstream proteomic analyses.

Protein-protein interaction studies: As a discrete region of KIAA0809, the peptide fragment is well-suited for probing potential protein-protein interactions that may be mediated by its C-terminal sequence. Researchers can employ the peptide in pull-down assays, surface plasmon resonance, or other binding studies to assess its capacity to interact with candidate binding partners identified through yeast two-hybrid screens or co-immunoprecipitation experiments. Such investigations provide mechanistic insights into the molecular networks involving KIAA0809 and its functional domains.

Phosphorylation and post-translational modification research: The 881-890 region may contain predicted or experimentally suggested sites for phosphorylation or other post-translational modifications. Synthetic access to this peptide enables the study of modification-specific antibodies or the creation of modified peptide analogs for in vitro kinase assays. By comparing modified and unmodified versions in biochemical assays, researchers can elucidate the regulatory significance of these modifications within the context of KIAA0809 function and signaling pathways.

Mass spectrometry reference standard: The defined sequence of the 881-890 peptide makes it a practical internal standard or calibration reference for mass spectrometry-based proteomic workflows. It can be spiked into complex biological samples to validate peptide identification, retention time, and quantification protocols during targeted or discovery-driven LC-MS/MS experiments. This use supports accurate and reproducible detection of KIAA0809-derived peptides in various biological matrices.

Functional domain analysis: By isolating the 881-890 fragment, investigators can systematically dissect the contribution of this specific region to the overall activity or structural stability of KIAA0809. Functional assays employing the peptide—such as competition experiments, structural modeling, or mutagenesis studies—can help clarify whether this segment is involved in critical functional motifs, protein folding, or localization signals. Such knowledge advances the understanding of KIAA0809's role in cellular physiology and its potential interactions within larger protein complexes.

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