L-HISTIDINE:HCL:H2O (D5; 15N3) is a hydrated hydrochloride salt of L-histidine in which the amino acid framework is isotopically labeled with deuterium (D5) and nitrogen-15 (15N3), retaining the histidine side chain that contains an imidazole functional group. The molecule bears an α-amino group and a α-carboxyl group, with the carboxylate present under salt conditions and the imidazole side chain capable of protonation/deprotonation depending on pH, while the hydrochloride and associated water define its solid-state salt form. This isotopically labeled amino acid is used as a tracer and analytical standard in isotope-labeling experiments, mass spectrometry and NMR method development, and in the preparation of labeled peptide or amino acid derivatives for structure-activity studies and biochemical binding or turnover assays where incorporation of the labeled histidine unit is required.
CAT No: L-Iso-0313
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