4-Nitro-Z-Gly-Trp-Gly-OH is a fluorogenic peptide substrate with a 4-nitro-phenyl group for detection, designed for use in protease assays. The substrate is cleaved by enzymes that hydrolyze peptide bonds between glycine and tryptophan residues. Researchers use it to monitor proteolytic activity and to identify enzyme inhibitors or substrates in high-throughput screenings.
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