Orexin B, rat, mouse is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively.
CAT No: R1577
CAS No:202801-92-1
Synonyms/Alias:Orexin B (mouse);202801-92-1;Orexin B (mouse, rat);Orexin B, rat, mouse TFA;C126H215N45O34S;HB5324;AKOS024456610;DA-56477;FO109203;PD079177;RPGPPGLQGRLQRLLQANGNHAAGILTM (modifications: Met-28 = C-terminal amide);H-Arg-Pro-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Asn-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr- Met-NH2; H-RPGPPGLQGR LQRLLQANGNHAAGILTM-NH2;
Orexin B, rat, mouse is a synthetic peptide that corresponds to the native Orexin B (also known as hypocretin-2) sequence found in both rat and mouse species. As a neuropeptide, it plays a pivotal role in the regulation of arousal, wakefulness, and energy homeostasis within the central nervous system. Its unique structure and receptor specificity make it highly relevant for investigating the orexinergic system, which has been implicated in a range of physiological and behavioral processes. The availability of this peptide in research-grade form enables detailed studies of its molecular mechanisms, receptor interactions, and downstream signaling pathways, thereby supporting advancements in neurobiology, pharmacology, and peptide-based assay development.
Neuropharmacology research: Orexin B is extensively utilized in neuropharmacological studies to elucidate the functional roles of orexin receptors, particularly the OX2R subtype. By applying the peptide to in vitro neuronal cultures or in vivo animal models, researchers can systematically characterize receptor activation, signal transduction events, and modulatory effects on neuronal excitability. These investigations are essential for understanding the molecular underpinnings of sleep-wake regulation, reward pathways, and stress responses, providing critical insights into the broader neurochemical landscape of the brain.
Peptide-receptor interaction studies: The rat and mouse Orexin B peptide serves as a valuable ligand for probing the binding dynamics and affinity profiles of orexin receptors. Through radioligand binding assays, fluorescence resonance energy transfer (FRET), or surface plasmon resonance (SPR) techniques, scientists can quantify the peptide's interaction with OX1R and OX2R, assess receptor selectivity, and map the structural determinants of peptide-receptor recognition. Such studies are instrumental in the rational design of receptor modulators and in advancing the field of structure-activity relationship (SAR) analysis for neuropeptides.
Behavioral neuroscience: The administration of Orexin B in rodent models enables detailed behavioral assessments related to arousal, feeding behavior, and locomotor activity. By manipulating peptide levels in specific brain regions, investigators can dissect the contribution of orexinergic signaling to circadian rhythms, motivation, and adaptive behaviors. These behavioral paradigms are widely used to explore the fundamental neurobiological mechanisms underpinning alertness, energy expenditure, and the integration of metabolic and environmental cues.
Electrophysiological investigations: The peptide is a powerful tool for studying synaptic transmission and neuronal network activity in brain slices or cultured neurons. Application of Orexin B allows researchers to monitor changes in membrane potential, firing rates, and synaptic plasticity, thereby elucidating the direct effects of orexinergic signaling on neuronal circuitry. Such electrophysiological analyses are crucial for mapping the connectivity and functional modulation of neural systems involved in vigilance, emotion, and cognitive processing.
Peptide assay development: Orexin B is frequently employed as a standard or control in the development and validation of bioanalytical assays targeting orexin peptides and their receptors. Its well-characterized sequence and activity profile facilitate the optimization of immunoassays, receptor binding assays, and mass spectrometry-based detection methods. These assay platforms are essential for high-throughput screening, quantitative analysis, and the identification of novel orexin pathway modulators in both academic and industrial research settings.
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