Ro 27-3225 TFA salt is a peptide derivative stabilized as a trifluoroacetate salt for improved handling. The sequence supports studies of folding, ligand-receptor interactions, and hydrophobic packing. Researchers use it to map structural determinants and assess biochemical behavior under controlled conditions. Its formulation enhances analytical consistency.
CAT No: PI-010
CAS No:1373926-49-8
Synonyms/Alias:RO27-3225 TFA;1373926-49-8;RO273225 TFA;RO27-3225 (TFA);MS-31674;RO 27-3225;(2S)-N-[(2S)-1-[(2-amino-2-oxoethyl)-methylamino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]-2-[[(2S)-2-[[(2S)-2-(butanoylamino)-3-(1H-imidazol-5-yl)propanoyl]amino]-3-phenylpropanoyl]amino]-5-(diaminomethylideneamino)pentanamide;2,2,2-trifluoroacetic acid;
Chemical Name:(2S)-N-[(2S)-1-[(2-amino-2-oxoethyl)-methylamino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]-2-[[(2S)-2-[[(2S)-2-(butanoylamino)-3-(1H-imidazol-5-yl)propanoyl]amino]-3-phenylpropanoyl]amino]-5-(diaminomethylideneamino)pentanamide
Ro 27-3225 TFA salt is a synthetic small molecule compound recognized for its role as a potent and selective inhibitor of protein kinase C (PKC) isoforms. As a research-use biochemical, it offers a valuable tool for dissecting PKC-mediated signal transduction pathways, which are pivotal in regulating diverse cellular processes such as proliferation, differentiation, and apoptosis. Its trifluoroacetate (TFA) salt form enhances solubility and stability, making it suitable for a wide range of laboratory applications. Owing to its high selectivity, Ro 27-3225 is frequently employed in studies aiming to elucidate the specific contributions of PKC family members in cellular signaling networks, providing researchers with a refined approach to modulate kinase activity and analyze downstream effects.
Signal transduction research: Ro 27-3225 TFA salt is widely utilized in the investigation of PKC-dependent signaling cascades. By selectively inhibiting PKC isoforms, researchers can delineate the specific roles of these kinases in transmitting extracellular signals to intracellular responses. This compound enables the dissection of complex signaling events, facilitating the identification of PKC substrates and the mapping of phosphorylation events that underpin cellular communication and regulatory mechanisms.
Cellular function analysis: The use of this inhibitor supports detailed studies into the influence of PKC activity on diverse cellular functions, including gene expression, cytoskeletal rearrangement, and cell cycle progression. By modulating PKC-driven pathways, scientists can probe the molecular basis of cellular behaviors and uncover mechanisms underlying processes such as migration, adhesion, and differentiation, thereby advancing the understanding of fundamental cell biology.
Pharmacological profiling: Ro 27-3225 TFA salt serves as a benchmark compound in the characterization of novel PKC modulators. Its defined selectivity profile allows for comparative studies, where new inhibitors or activators are evaluated for potency, specificity, and mechanism of action. This application is critical in drug discovery programs focused on kinase-targeted therapeutics, as it provides a reference standard for assessing the efficacy and selectivity of experimental compounds.
Disease mechanism studies: The compound is instrumental in preclinical research aimed at elucidating the involvement of PKC isoforms in pathological conditions such as cancer, neurodegeneration, and cardiovascular dysfunction. By inhibiting PKC activity in relevant cellular or animal models, investigators can assess the contribution of specific signaling pathways to disease progression, offering insights into molecular targets for future therapeutic intervention.
Biochemical assay development: Ro 27-3225 TFA salt is employed in the optimization and validation of in vitro kinase assays designed to measure PKC activity. Its application as a selective inhibitor aids in establishing assay specificity, determining dynamic range, and confirming that observed effects are attributable to PKC modulation. Such rigorous assay validation is essential for generating reliable, reproducible data in both academic and industrial research settings, thereby supporting the advancement of kinase biology and inhibitor development.
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