10Panx is a mimetic inhibitory peptide of panx1, which blocks pannexin-1 gap junctions, inhibits p2x7-mediated dye uptake, atp-mediated il-1β release and caspase-1 activation, but does not alter membrane current in macrophages in vitro.
CAT No: R0961
CAS No:955091-53-9
Synonyms/Alias:10PANX;955091-53-9;10 Panx;10Panx1;H-Trp-Arg-Gln-Ala-Ala-Phe-Val-Asp-Ser-Tyr-OH;CHEMBL5439198;Panx-1 mimetic inhibitory peptide;HY-P1137;AKOS024457570;DA-49060;PD079477;CS-0028050;
10Panx is a synthetic peptide compound specifically designed as a selective inhibitor of pannexin-1 (Panx1) channels. As a mimetic peptide corresponding to the first extracellular loop of Panx1, it has become a valuable tool for dissecting the physiological and pathological roles of pannexin-mediated signaling in cellular communication. Its high specificity and reversible binding properties have made it a prominent reagent in the study of purinergic signaling, inflammasome activation, and intercellular ATP release. By enabling targeted modulation of Panx1 channel activity, 10Panx supports diverse research applications across neuroscience, immunology, and cell biology.
Ion Channel Research: In electrophysiological studies, 10Panx serves as a precise molecular probe for investigating the biophysical properties and gating mechanisms of pannexin-1 channels. Researchers utilize the peptide to selectively inhibit Panx1-mediated ionic currents in patch-clamp experiments, allowing for the isolation of Panx1-specific conductance from overlapping channel activities. This targeted approach aids in elucidating the contribution of pannexin channels to overall membrane permeability and electrical signaling in excitable and non-excitable cells.
Purinergic Signaling Studies: The peptide is extensively used to examine the role of Panx1 in ATP release and subsequent purinergic receptor activation. By blocking Panx1 channels, 10Panx enables controlled experiments that distinguish between pannexin-dependent and independent pathways of extracellular nucleotide signaling. Such studies are critical for understanding how ATP release via Panx1 influences autocrine and paracrine signaling processes in immune cells, neurons, and glial populations.
Inflammasome Activation Assays: 10Panx is frequently employed in immunological research to assess the involvement of pannexin-1 channels in inflammasome assembly and activation. Its inhibitory action allows for the differentiation of Panx1-mediated ATP release and subsequent NLRP3 inflammasome activation from alternative mechanisms. This application is particularly relevant in studies aiming to unravel the molecular underpinnings of sterile inflammation, cytokine maturation, and innate immune responses.
Neurobiological Investigations: In the context of neuroscience, the peptide is utilized to probe the contribution of Panx1 channels to neuronal excitability, synaptic transmission, and neuroinflammatory processes. Experimental models employing 10Panx facilitate the analysis of pannexin-dependent modulation of neurotransmitter release, glial cell signaling, and neuronal survival. These insights are instrumental in advancing the fundamental understanding of central nervous system physiology and the role of pannexins in neurodegenerative conditions.
Cellular Communication and Death Pathways: Researchers also deploy 10Panx to investigate the role of pannexin-1 channels in mediating intercellular communication and regulated cell death. By selectively inhibiting Panx1, the peptide allows for the assessment of its involvement in processes such as apoptosis, pyroptosis, and the propagation of damage signals. This application is particularly valuable for delineating the contribution of pannexin-mediated ATP release to cell fate decisions and tissue homeostasis in various experimental systems.
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