(4S)-4,5-Bis-[2-((RS)-1-ethoxy-ethylsulfanyl)-acetamido]-pentanoic acid-2,3,5,6-tetrafluoro-phenyl ester is a fluorinated, multi-substituted amino acid derivative featuring a pentanoic acid backbone bearing two acetamido substituents and an aryl ester linkage to a 2,3,5,6-tetrafluorophenyl group. The molecule contains an amino functionality within the acetamido groups and a carboxyl group converted to a phenyl ester, while the side-chain includes thioether sulfanyl substituents with an explicitly indicated (RS) configuration and the backbone stereocenter is specified as (4S). In chemical biology and peptide chemistry workflows, this protected/derivatized amino acid ester format provides a defined, fluorinated and thioether-bearing building block for preparing more complex amino acid and peptide derivatives, including labeled or structurally constrained analogues for structure-activity studies and analytical method development.
CAT No: CP26266
CAS No:125488-70-2
Synonyms/Alias:4,5-Di-[S-(1-ethoxyethyl)mercaptoacetamido]pentanoic acid-2,3,5,6-tetrafluorophenyl ester;(4S)-4,5-Bis-[S-(1-ethoxyethyl)-mercaptoacetamido]-pentanoic acid-2,3,5,6-tetrafluorophenyl ester
(4S)-4,5-Bis-[2-((RS)-1-ethoxy-ethylsulfanyl)-acetamido]-pentanoic acid-2,3,5,6-tetrafluoro-phenyl ester is a fluorinated, esterified amino-acid derivative designed for chemical biology and analytical workflows where a defined aromatic handle and multiple amide functionalities are advantageous. The molecule contains a stereodefined (4S) amino-acid backbone bearing bis-acetamide substituents, along with a tetrafluoro-phenyl ester group that supports robust, nonpolar-to-intermediate polarity behavior in derivatization and capture strategies. The presence of thioether-containing side functionalities further enables selective downstream transformations and conjugation-like chemistry in research settings.
1. Chemical Biology Probes
(4S)-4,5-Bis-[2-((RS)-1-ethoxy-ethylsulfanyl)-acetamido]-pentanoic acid-2,3,5,6-tetrafluoro-phenyl ester is used as a probe scaffold in chemical biology programs that require a fluorinated aromatic motif and multiple amide linkages for stable probe architecture. Researchers leverage this structure when building small-molecule probes that can be incorporated into broader labeling or detection workflows, where the tetrafluoro-phenyl ester provides a distinct chemical signature for downstream handling and analytics. The bis-acetamide pattern supports controlled presentation of functional groups, while the thioether-containing substituents provide a practical handle for further derivatization steps during probe optimization.
2. Proteomics And LC-MS Workflows
(4S)-4,5-Bis-[2-((RS)-1-ethoxy-ethylsulfanyl)-acetamido]-pentanoic acid-2,3,5,6-tetrafluoro-phenyl ester is applied in proteomics-adjacent analytical workflows as a defined amino-acid-based chemical standard or derivatization component where a strongly characteristic fluorinated aromatic group improves traceability. Analytical chemists and mass spectrometry method developers commonly select fluorinated building blocks to create unambiguous mass/fragmentation features and to reduce ambiguity in complex mixtures. The ester and amide-rich framework supports consistent chemical behavior during sample preparation steps, enabling reproducible readouts when the compound is used to validate derivatization performance, retention behavior, or instrument response.
3. Pharmaceutical Intermediate Development
(4S)-4,5-Bis-[2-((RS)-1-ethoxy-ethylsulfanyl)-acetamido]-pentanoic acid-2,3,5,6-tetrafluoro-phenyl ester is used as an advanced intermediate building block in medicinal-chemistry and specialty synthesis programs that require a stereodefined amino-acid derivative with a fluorinated phenyl ester motif. Process and R&D chemists employ this type of scaffold to assemble larger fragments where the tetrafluoro-phenyl group serves as a useful structural element and a controlled functional handle for subsequent synthetic elaboration. The bis-amide substitution pattern provides multiple sites for downstream functional group management, supporting structured diversification in lead optimization campaigns and analog synthesis.
4. Targeted Derivatization Reagents
(4S)-4,5-Bis-[2-((RS)-1-ethoxy-ethylsulfanyl)-acetamido]-pentanoic acid-2,3,5,6-tetrafluoro-phenyl ester is also used as a targeted derivatization reagent component in analytical chemistry when a stable, well-defined amino-acid derivative is needed to introduce a fluorinated aromatic tag. Researchers choose this scaffold to improve detectability and to create consistent derivatives for method development, including studies focused on sample cleanup, enrichment, or comparative quantitation across conditions. The combination of ester functionality and multiple acetamide groups helps maintain a predictable chemical identity through preparation steps, while the thioether-containing substituents provide a practical basis for further transformation or coupling in workflow-specific derivative schemes.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More