Ac-Ile-NHMe is an N-acetylated amino acid amide derivative of isoleucine, featuring the isoleucine side chain attached to a peptide-like backbone terminated as a methylamide (-NHMe) rather than a free carboxylic acid. The molecule contains an acetyl group on the amino terminus (-NHAc) and an amide linkage at the C-terminus (-C(=O)NHMe), with the isoleucine β-branched alkyl side chain providing hydrophobic character for conformational and intermolecular interactions. Ac-Ile-NHMe is used as a defined amino acid building block or model substrate in peptide chemistry and structure-property studies, including studies of amide-containing fragments, analytical standards for LC/UV or MS method development, and synthesis of more complex acylated or amidated peptide intermediates.
CAT No: CP27000
CAS No:32483-16-2
Synonyms/Alias:32483-16-2;Ac-Ile-NHMe;Acetyl-L-isoleucinemethylamide;SCHEMBL13596361;CTK8F7539;ZINC2560833;AKOS006274979
Ac-Ile-NHMe is an N-acetylated isoleucine amide (Ac-Ile-NHMe) that provides a protected, peptide-like backbone with an amide-linked C-terminus, making it a stable, non-ionizable building block for chemical and analytical workflows. The isoleucine side chain retains the characteristic hydrophobic, branched aliphatic functionality, while the N-acetyl group and terminal N-methylamide cap suppress free amino and carboxyl reactivity, respectively. This combination is commonly used as a defined small-molecule substrate mimic for studying peptide recognition chemistry and for preparing standards in peptide/amide-focused analytical methods.
1. Peptide Substrate Mimics
Ac-Ile-NHMe is used in chemical biology and enzymology-adjacent research as a minimal peptide surrogate to probe peptide bond recognition, acyl-terminus effects, and structure-dependent substrate preferences in model systems. Researchers employ this capped isoleucine amide to reduce confounding variables associated with free termini, enabling cleaner comparisons across related Ile-containing analogs. The N-acetyl and C-terminal N-methylamide pattern helps maintain a peptide-like electronic environment while keeping the molecule compact for routine handling in screening and assay development.
2. Amide-Bond Analytical Standards
Ac-Ile-NHMe serves as a defined reference standard for LC-MS and related analytical workflows that target peptide fragments, acylated amino acid amides, or isoleucine-containing amide species. Because both termini are capped (acetylated N-terminus and N-methylamide C-terminus), the compound behaves as a stable, reproducible analyte for method qualification, calibration, and retention-time mapping. Analytical method developers and proteomics/peptidomics teams use such standards to verify identification of small peptide-like components and to support quantitative workflows where fragment-level consistency matters.
3. Peptidomimetic Building Block
Ac-Ile-NHMe is applied as a small, peptide-mimicking building block in peptidomimetic and medicinal chemistry intermediate design where a capped isoleucine residue is required without introducing additional reactive functional groups. Medicinal chemistry groups use it to construct constrained or simplified amide architectures that emulate local peptide geometry while improving synthetic tractability and handling. The retained branched hydrophobic side chain supports SAR studies that differentiate isoleucine-like steric profiles from other aliphatic residues in fragment-based analog series.
4. Hydrophobic Fragment Libraries
Ac-Ile-NHMe is also used to assemble or evaluate hydrophobic fragment libraries and small-molecule libraries that incorporate isoleucine-derived amide motifs. Chemical biology and discovery chemistry teams often select capped amino acid amides to standardize backbone electronics and terminal protection, allowing side-chain identity to drive observed behavior in downstream screening readouts. In these library workflows, the molecule's peptide-like amide functionality and branched aliphatic side chain provide a consistent fragment scaffold for parallel synthesis and comparative characterization.
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