Biotinyl-Ala-Ser-Thr-DL-Asp-fluoromethylketone features a biotinylated peptide backbone terminating in a fluoromethylketone warhead. The electrophilic group can form covalent adducts with catalytic residues in protease active sites. Its design allows visualization and pull-down of enzyme complexes via biotin affinity. Research applications include active-site labeling, mechanistic enzymology, and target-identification studies.
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