Boc-LGR-pNA, a chromogenic substrate for horseshoe crab clotting enzyme, which is used in quantitative assays of endotoxin.
CAT No: 10-101-349
CAS No:68223-96-1
Synonyms/Alias:ENDOTOXIN SUBSTRATE;68223-96-1;tert-butyl N-[(2S)-1-[[2-[[(2S)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-4-methyl-1-oxopentan-2-yl]carbamate;SCHEMBL3396260;tert-Butyl ((S)-1-((2-(((S)-5-guanidino-1-((4-nitrophenyl)amino)-1-oxopentan-2-yl)amino)-2-oxoethyl)amino)-4-methyl-1-oxopentan-2-yl)carbamate;
Chemical Name:tert-butyl N-[1-[[2-[[5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-4-methyl-1-oxopentan-2-yl]carbamate
Boc-Leu-Gly-Arg-pNA, Acetate is a synthetic chromogenic peptide substrate widely utilized in biochemical and molecular biology research. Featuring a protected N-terminus with a tert-butyloxycarbonyl (Boc) group and a para-nitroanilide (pNA) moiety at the C-terminus, this compound is designed for sensitive detection and quantification of specific proteolytic activities. Its unique sequence, incorporating leucine, glycine, and arginine, allows researchers to investigate protease specificity and catalytic mechanisms. The acetate salt form enhances its solubility and stability, making it suitable for a variety of in vitro experimental settings. As a versatile reagent, Boc-Leu-Gly-Arg-pNA enables precise monitoring of enzymatic reactions by producing a chromogenic signal upon cleavage, which can be easily measured spectrophotometrically.
Protease Activity Assays: Boc-Leu-Gly-Arg-pNA, Acetate is extensively used as a substrate to evaluate the activity of serine proteases, particularly those with a preference for arginine-containing peptide bonds. When cleaved by target enzymes, the pNA group is released, resulting in a measurable yellow color that provides a direct readout of enzymatic activity. This property makes it an indispensable tool for enzyme kinetics studies, inhibitor screening, and the functional characterization of novel proteases in both academic and industrial laboratories.
Enzyme Kinetics Research: In enzyme kinetics, this chromogenic substrate enables researchers to determine critical parameters such as Km and Vmax for proteolytic enzymes. By monitoring the rate of pNA release under varying substrate concentrations, scientists can gain insights into enzyme-substrate interactions, catalytic efficiency, and the effects of potential modulators. Its well-defined sequence and robust chromogenic response facilitate reproducible and quantitative analyses, supporting the development of mechanistic models and the validation of theoretical predictions.
High-Throughput Screening: The use of Boc-Leu-Gly-Arg-pNA in high-throughput screening platforms accelerates the discovery of protease inhibitors and activators. Its compatibility with microplate readers allows for rapid and parallel processing of numerous samples, significantly increasing throughput and data quality. This approach is particularly valuable in pharmaceutical research, where large compound libraries are screened to identify lead molecules that modulate protease function, ultimately contributing to the development of new therapeutic strategies.
Biochemical Pathway Elucidation: Researchers employ this substrate to dissect complex proteolytic cascades involved in physiological and pathological processes. By selectively monitoring the activity of specific enzymes within a pathway, it becomes possible to map sequence-specific cleavage events and identify regulatory nodes. The chromogenic output enables real-time tracking of proteolytic events, offering a dynamic view of enzyme function under various experimental conditions.
Quality Control in Enzyme Production: In industrial biotechnology, Boc-Leu-Gly-Arg-pNA, Acetate is used to assess the potency and consistency of protease preparations during manufacturing. By providing a quantitative measure of enzyme activity, it helps ensure batch-to-batch reproducibility and supports process optimization. Its straightforward assay protocol and reliable performance make it a standard reagent for quality assurance in enzyme production pipelines.
Peptide substrate Boc-Leu-Gly-Arg-pNA, Acetate continues to play a pivotal role in advancing protease research, enzyme characterization, and the development of biochemical assays. Its adaptability to diverse experimental formats, including spectrophotometric and high-throughput applications, underscores its value across multiple scientific disciplines. By enabling precise and efficient measurement of proteolytic activity, this substrate supports innovations in basic research, drug discovery, and industrial biotechnology, cementing its status as a fundamental tool in the study of protease function and regulation.
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