Coiled-coil domain-containing protein 110 (770-778)

Coiled-coil domain-containing protein 110 (770-778) is a synthetic peptide fragment derived from the larger CCD110 protein, which is characterized by its distinctive coiled-coil structural motif. Peptides encompassing coiled-coil domains are widely recognized for their role in mediating protein-protein interactions and contributing to the structural integrity of various cellular components. The 770-778 sequence represents a specific region of the CCD110 protein, making it a valuable tool for researchers investigating the functional properties, interaction dynamics, and structural features of coiled-coil domains within the context of cellular biology and protein engineering.

Protein-Protein Interaction Studies: As a defined peptide segment from a coiled-coil domain, this compound is ideally suited for dissecting the molecular basis of protein-protein interactions. Researchers can employ it in binding assays, such as pull-down or surface plasmon resonance experiments, to characterize the affinity and specificity of interactions mediated by the CCD110 coiled-coil motif. Such investigations facilitate a deeper understanding of how coiled-coil domains contribute to the assembly and regulation of multi-protein complexes, with implications for elucidating cellular architecture and signaling pathways.

Structural Biology Research: The peptide serves as a model system for examining the structural determinants of coiled-coil formation. By utilizing techniques such as circular dichroism spectroscopy, nuclear magnetic resonance, or X-ray crystallography, scientists can probe the secondary structure, stability, and folding behavior of the 770-778 region. These insights are essential for mapping the conformational landscape of coiled-coil domains and for informing the rational design of synthetic peptides or engineered proteins with tailored structural properties.

Peptide-Based Inhibitor Design: The defined sequence of this peptide fragment enables its use as a template in the development of inhibitors targeting coiled-coil-mediated interactions. By mimicking or competitively binding to partner proteins, the peptide can help identify critical contact residues and inform the design of molecules that modulate the assembly of protein complexes. This approach is particularly valuable in the context of pathway dissection, where selective perturbation of specific interactions is required to clarify functional roles within complex cellular networks.

Antibody Generation and Epitope Mapping: The 770-778 peptide fragment can be utilized as an antigen for generating sequence-specific antibodies or for mapping antibody binding sites within the CCD110 protein. Such antibodies are important reagents in immunodetection assays, including Western blotting, immunoprecipitation, and immunofluorescence, where precise recognition of the coiled-coil domain is required. The peptide's defined sequence ensures high specificity, supporting the development of reliable immunological tools for protein localization and interaction studies.

Peptide Array and Functional Screening: The synthetic nature of the peptide allows it to be incorporated into high-throughput peptide arrays for systematic screening of binding partners, post-translational modification sites, or functional motifs within the CCD110 coiled-coil domain. These array-based approaches accelerate the identification of novel interaction networks and regulatory mechanisms, providing a platform for hypothesis-driven research into the roles of coiled-coil domains in cellular processes and protein assembly.

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