Fmoc-Ala-OH-1-13C is an Fmoc-protected alanine derivative bearing a carboxylic acid and an isotopic label at the 13C position indicated in the product name, making it an isotopically labeled, protected amino acid suitable for peptide-related synthesis. The molecule contains the Fmoc (9H-fluorenylmethoxycarbonyl) protecting group on the amino functionality, while the side chain corresponds to alanine's methyl group and the free carboxyl group remains available for coupling chemistry. In research workflows such as solid-phase or solution-phase peptide synthesis and isotopic labeling for NMR or mass spectrometry-based analytical studies, this compound functions as a labeled building block for incorporating the 13C-enriched alanine residue into defined peptide or amino acid derivative structures.
Fmoc-Ala-OH-1-13C is an Fmoc-protected alanine building block bearing a stable 13C label at the alanine carbon position relevant to alanine-derived fragments. As a labeled, protected amino acid suitable for peptide assembly, it supports downstream workflows where the isotopic tag is used to track incorporation and to generate predictable mass shifts for LC-MS quantification or structural verification. Researchers commonly select this format when they need an alanine residue that is compatible with Fmoc-based peptide synthesis while providing an isotopic signature for analytical readouts.
1. Isotopically Labeled Peptide Synthesis
Fmoc-Ala-OH-1-13C is used to prepare peptides and peptide standards that contain a defined 13C-labeled alanine residue, enabling controlled incorporation of the isotopic tag during custom peptide synthesis. Peptide chemists and contract peptide manufacturers use this building block to generate internal reference peptides for method development, to support comparative studies where alanine-containing sequences must be distinguished by mass, and to confirm sequence-specific incorporation in labeled constructs. The Fmoc-protected format aligns with routine solid-phase peptide synthesis workflows, while the single 13C label provides a straightforward, interpretable mass contribution without introducing additional reactive functionality.
2. LC-MS Quantification Standards
Fmoc-Ala-OH-1-13C is frequently selected for quantitative LC-MS workflows that require isotopically shifted peptide species as calibration materials or internal standards. Analytical chemists use labeled alanine-containing peptides to improve quantitation robustness by accounting for variability in extraction, ionization, and instrument response, particularly in studies that monitor alanine-bearing fragments or alanine-substituted motifs. Because the isotopic label is embedded in the peptide backbone through the labeled amino acid building block, the resulting mass shift is directly tied to the target residue, supporting cleaner discrimination between closely related peptide forms.
3. Protein/Peptide Structural Verification
Fmoc-Ala-OH-1-13C supports structural characterization of peptide products and protein digests where alanine-containing segments need unambiguous assignment. Protein science groups use isotopically labeled peptides to aid interpretation of MS/MS spectra, to validate peptide identity in complex mixtures, and to resolve ambiguity when multiple sequence candidates produce similar fragment patterns. The Fmoc-protected, residue-level labeling strategy helps ensure that the isotopic signature is localized to the alanine position, which is particularly useful when confirming site-specific incorporation in engineered peptide constructs or when benchmarking analytical pipelines for sequence confirmation.
4. Metabolic and Flux Tracing Tools
Fmoc-Ala-OH-1-13C is applied as a labeled reference material in metabolic research contexts where alanine-derived species are analyzed by mass spectrometry. Metabolomics and isotope-tracing researchers use isotopically defined alanine-containing peptides or peptide fragments as comparators to interpret labeled-to-unlabeled distributions, to validate extraction and digestion workflows, and to support data processing for isotope-resolved readouts. This use is driven by the stable 13C label's compatibility with MS-based detection and by the ability to generate reproducible labeled reference structures through Fmoc-based peptide assembly.
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