L-ALANINE (3-13C; 2-D) is an isotopically labeled amino acid belonging to the aliphatic, nonpolar alanine family, featuring a methyl side chain attached to the α-carbon and an amino group and a carboxyl group on the same carbon framework. The molecule bears a 13C label at the 3-position of the alanine carbon skeleton and deuterium at the 2-position, with stereochemistry consistent with the "L" designation and retaining the free amino acid functional group pattern (NH2 and CO2H). It is used in isotopic tracing, quantitative LC-MS or NMR method development, and structure-property or metabolic labeling studies where incorporation of alanine with defined isotopic enrichment supports analytical discrimination of labeled species and related amino acid derivatives.
CAT No: L-Iso-0038
L-ALANINE (3-13C; 2-D) is an isotopically labeled alanine reagent bearing a 13C label at the C-3 position and deuterium at the C-2 position, enabling mass-shifted tracking of alanine in analytical and tracer workflows. This stable isotope labeling pattern is commonly leveraged to distinguish labeled alanine from endogenous alanine and to support quantitative LC-MS or GC-MS measurements where alanine isotopologues are resolved. Researchers use this form as a defined isotopic standard or tracer component in studies that require controlled incorporation of alanine labeling into downstream analytical readouts.
1. Metabolomics Internal Standard
L-ALANINE (3-13C; 2-D) is frequently used as an internal standard for quantitative metabolomics and targeted amino acid profiling, where the labeled alanine provides a reliable reference for extraction efficiency, ionization variability, and instrument response. Metabolic panels that quantify alanine alongside other amino acids benefit from this reagent because the 13C and deuterium combination produces a distinct mass signature, improving confidence in peak assignment and reducing interference from unlabeled alanine. Analytical method development teams and metabolomics laboratories commonly include isotopically labeled amino acids like this one to normalize LC-MS or GC-MS results and to support robust calibration strategies for amino acid quantitation.
2. Stable Isotope Tracing Studies
L-ALANINE (3-13C; 2-D) is applied in stable isotope tracing experiments to follow the fate of alanine-derived carbon and labeled hydrogen through biochemical workflows and downstream analytical detection. Metabolic research groups use this tracer to monitor incorporation patterns into metabolites that reflect alanine utilization and related metabolic flux, with the specific 13C placement at C-3 and deuterium at C-2 providing measurable isotopic enrichment in mass-based readouts. This reagent is particularly useful when researchers need a defined alanine isotopologue to interpret labeling patterns in complex biological matrices, including cell extracts and metabolite mixtures analyzed by LC-MS.
3. Targeted LC-MS Quantification
L-ALANINE (3-13C; 2-D) supports targeted quantitative LC-MS applications where alanine is measured with high specificity using isotopic mass differences rather than relying on retention-time matching alone. Bioanalytical method developers and proteomics/metabolomics instrument users often select this labeled alanine to generate calibration curves, assess linearity, and verify assay performance for amino acid quantification in biological or chemical samples. The combined 13C and deuterium labeling helps produce a clear isotopologue separation from the native amino acid signal, which is valuable for experiments that demand accurate quantitation under conditions where unlabeled alanine may be abundant.
4. Amino Acid Standard Calibration
L-ALANINE (3-13C; 2-D) is routinely used as a defined isotopic standard for calibration and qualification of analytical workflows that quantify amino acids, including amino acid derivatization-based methods coupled to mass spectrometry. Analytical chemistry teams use this reagent to confirm mass accuracy, evaluate recovery and reproducibility, and ensure consistent performance across instrument runs and sample batches. Because the isotopic composition is fixed and traceable, it is also used to standardize comparisons between experiments where alanine quantitation must be consistent, such as in method transfer studies or longitudinal analytical monitoring of labeled/unlabeled sample sets.
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