L-ALANINE (3-13C) is an isotopically labeled, naturally occurring amino acid derivative in which the alanine side chain bears a 13C label at the carbon position corresponding to the methyl carbon (C-3) while the molecule retains the amino acid backbone and L-stereochemical form indicated by the name. The compound contains a primary amino group and a carboxyl group, and its side chain is a nonpolar methyl substituent, enabling it to participate in standard amino acid handling and peptide-coupling chemistry as an alanine building block. In biochemical and analytical research, the 13C label supports isotopic tracing, NMR/IR/HRMS-based quantitation, and incorporation studies where carbon-specific labeling of alanine-derived fragments is used to follow labeling patterns in peptides, proteins, or metabolic transformation experiments.
CAT No: L-Iso-0037
L-ALANINE (3-13C) is an L-alanine stable isotope-labeled building block in which the carbon at the 3-position of the alanine side chain is enriched with 13C. As an isotope-labeled amino acid, it is widely used as a quantitative tracer and reference material for mass spectrometry and related analytical workflows, where the predictable 13C mass shift enables confident identification and measurement. Researchers select this specific labeling pattern to track alanine-derived carbon signals and to support isotopically resolved calibration or internal standard strategies in metabolomics, proteomics, and biochemical quantification studies.
1. Metabolomics Carbon Tracing
L-ALANINE (3-13C) is used in metabolic flux and metabolite profiling experiments to follow labeled carbon incorporation and redistribution involving alanine pools. Metabolic research groups commonly employ this tracer in LC-MS workflows where the 13C label at the side-chain position produces a clear, monitorable isotopologue signal, supporting time-course and condition-dependent comparisons. The defined enrichment at the 3-position helps distinguish alanine-derived carbon contributions from unlabeled background, improving interpretability in studies that quantify labeled metabolite fractions rather than total concentration alone.
2. Quantitative LC-MS Internal Standard
L-ALANINE (3-13C) serves as a robust isotope-resolved reference for quantitative amino acid analysis by LC-MS, including targeted metabolomics panels and amino acid quantification methods. Analytical chemistry teams use labeled alanine to correct for sample-to-sample variability in extraction efficiency, ionization, and instrument response, leveraging the close chemical identity between labeled and unlabeled alanine while maintaining a distinct mass signature. This makes the 13C-labeled compound particularly useful for calibration and normalization strategies where accurate, reproducible quantitation of alanine and related isotopologues is required.
3. Proteomics and Amino Acidomics Standards
L-ALANINE (3-13C) is also applied as a standard material in protein and peptide quantification workflows that require isotopic resolution of alanine-containing fragments or amino acid-derived signals. Proteomics and amino acidomics laboratories use 13C-labeled alanine to support method development, verification of labeling/derivatization consistency, and quality control of analytical runs that rely on mass-based detection. The single-site 13C labeling provides a controlled mass shift that can be exploited for identifying and quantifying alanine-associated features in complex biological matrices.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More