p14ARF-ORF3
p14ARF-ORF3 (125-133) is a synthetic peptide derived from the carboxyl-terminal region of the p14ARF protein, a well-characterized tumor suppressor involved in cell cycle regulation and cellular stress responses. This nonapeptide encompasses amino acids 125 to 133 of the open reading frame 3 (ORF3) of p14ARF, providing a defined sequence motif commonly utilized in molecular biology and biochemistry research. Its structural features and functional residues make it a valuable research tool for exploring protein-protein interactions, post-translational modifications, and cellular signaling mechanisms associated with the ARF pathway. As a chemically synthesized peptide, it offers precise sequence control and reproducibility for a variety of in vitro experimental applications.
Protein interaction studies: The p14ARF-ORF3 (125-133) peptide is widely used in assays designed to map and characterize protein-protein interactions involving the ARF tumor suppressor. By providing a defined epitope corresponding to a biologically significant region, it enables researchers to probe binding affinities, specificity, and interaction domains with ARF-binding partners, such as MDM2 or nucleophosmin. Pull-down assays, surface plasmon resonance, and co-immunoprecipitation experiments can leverage this peptide to elucidate the molecular mechanisms by which ARF modulates cellular pathways.
Antibody epitope mapping: Due to its sequence specificity, this peptide serves as a standard reagent for mapping antibody binding sites and validating the specificity of custom or commercial antibodies raised against p14ARF. By using the peptide in ELISA, western blot, or immunoprecipitation protocols, investigators can distinguish between antibodies that recognize linear versus conformational epitopes, thereby improving the reliability and interpretability of immunodetection assays targeting ARF or its fragments.
Post-translational modification analysis: The defined sequence of p14ARF-ORF3 (125-133) makes it a suitable substrate for in vitro studies of post-translational modifications, including phosphorylation, acetylation, or ubiquitination. Researchers can utilize the peptide in kinase assays or other enzymatic modification systems to identify modification sites, assess enzyme specificity, and investigate the functional consequences of such modifications on ARF signaling. Synthetic peptides like this one allow for controlled experimentation that is not confounded by the complexity of full-length proteins.
Cellular signaling pathway research: As a functional motif of the ARF protein, this peptide can be used to dissect signaling pathways implicated in cell cycle arrest, apoptosis, and tumor suppression. By introducing the peptide into cell-free systems or as a competitor in binding assays, scientists can investigate the downstream effects of ARF pathway modulation, providing insights into the regulation of p53 and other key effectors. Such studies contribute to a deeper understanding of cellular stress responses and oncogenic transformation mechanisms.
Peptide-based assay development: The chemical stability and defined composition of p14ARF-ORF3 (125-133) support its use as a standard or control reagent in the development of peptide-based assays. It can be incorporated into high-throughput screening platforms, biosensor calibration, or quality control protocols where precise quantification and reproducibility are essential. Its application in assay development facilitates the optimization of detection methodologies and the establishment of robust experimental workflows for ARF-related research.
Through these diverse applications, the p14ARF-ORF3 (125-133) peptide serves as a versatile and reliable tool for advancing molecular and cellular research focused on the ARF tumor suppressor pathway and its associated biological processes.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More