Pep-1 (uncapped) is a cell-penetrating peptide.
CAT No: R1606
CAS No:395069-86-0
Synonyms/Alias:Pep-1 uncapped;395069-86-0;Pep-1 (uncapped);AKOS040764099;DA-66574;TS-10466;
Pep-1 uncapped is a synthetic peptide known for its role as a cell-penetrating peptide (CPP), widely utilized in biochemical and molecular biology research. Characterized by its amphipathic sequence, Pep-1 uncapped facilitates the non-covalent delivery of a broad range of biomolecules—including proteins, peptides, and nucleic acids—across cellular membranes. Its uncapped structure enhances its interaction with cargo molecules and cellular surfaces, making it a versatile tool for investigating intracellular delivery mechanisms, protein transduction, and cellular uptake pathways. As a result, Pep-1 uncapped has become a valuable resource for scientists aiming to explore and manipulate cellular processes in vitro.
Intracellular delivery studies: As a robust cell-penetrating peptide, Pep-1 uncapped is extensively employed to mediate the efficient transport of bioactive macromolecules into live cells. Researchers leverage its ability to form stable, non-covalent complexes with proteins or nucleic acids, enabling functional delivery without the need for chemical modification of the cargo. This application is crucial for examining the intracellular actions of exogenous proteins, studying signal transduction pathways, and evaluating the cellular effects of delivered peptides or oligonucleotides under controlled experimental conditions.
Protein transduction research: The unique amphipathic structure of Pep-1 uncapped allows it to interact favorably with both hydrophobic and hydrophilic regions of target proteins, promoting their cellular uptake. This property is particularly valuable in investigations that require the introduction of recombinant proteins, enzymes, or antibody fragments directly into the cytosol or nucleus. By bypassing endosomal entrapment and facilitating rapid cytoplasmic delivery, Pep-1 uncapped supports studies focused on protein localization, functional complementation assays, and the elucidation of protein-protein interactions within living cells.
Peptide-functional studies: Owing to its efficient membrane translocation capability, Pep-1 uncapped serves as an essential tool for examining the biological activities of synthetic or modified peptides inside cells. Scientists utilize it to deliver regulatory peptides, signaling motifs, or peptide inhibitors to dissect cellular pathways, modulate gene expression, or probe receptor-mediated processes. This approach enables precise functional characterization of peptide-based effectors in a physiologically relevant context, advancing the understanding of intracellular signaling networks and peptide-target interactions.
Live-cell imaging and tracking: The peptide's ability to transport fluorescently labeled proteins and probes into intact cells under physiological conditions enhances live-cell imaging applications. Researchers can employ Pep-1 uncapped to introduce imaging agents, biosensors, or reporter constructs, allowing real-time visualization of dynamic cellular events. This application is instrumental for time-lapse microscopy, subcellular localization studies, and tracking the intracellular fate of delivered biomolecules, thereby providing valuable insights into cellular dynamics and molecular trafficking.
Assay development and screening: In high-throughput screening and assay development, Pep-1 uncapped offers a reliable platform for the delivery of functional biomolecules directly into assay systems. Its use facilitates the rapid assessment of compound activity, target engagement, or pathway modulation in cell-based assays without the confounding effects of transfection reagents or viral vectors. This capability streamlines the evaluation of candidate molecules, supports phenotypic screening, and accelerates the identification of modulators in drug discovery or functional genomics research.
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