A peptide containing residues 45-60 of the guanine nucleotide exchange factor (GEF) recognition/activation site of Rac1, which is specific inhibitor of Rac1-GEF interaction.
CAT No: R0907
CAS No:1095179-01-3
Synonyms/Alias:Rac1 Inhibitor W56;1095179-01-3;MVDGKPVNLGLWDTAG;CHEBI:167451;AKOS024456997;PD079275;S-1095179-01-3;L-methionyl-L-valyl-L-alpha-aspartyl-glycyl-L-lysyl-L-prolyl-L-valyl-L-asparagyl-L-leucyl-glycyl-L-leucyl-L-tryptophyl-L-alpha-aspartyl-L-threonyl-L-alanyl-glycine;
Rac1 Inhibitor W56 is a synthetic small molecule that specifically targets Rac1, a member of the Rho family of small GTPases, which plays a pivotal role in the regulation of cytoskeletal dynamics, cell migration, and intracellular signaling pathways. As a highly selective inhibitor, W56 is widely recognized for its utility in dissecting the molecular mechanisms governed by Rac1 activity in diverse cellular contexts. Researchers value its ability to modulate Rac1-dependent processes, making it a powerful tool for studying fundamental aspects of cell biology, cancer research, and signal transduction. The compound's biochemical specificity and reversible mode of action provide a robust experimental platform for investigating Rac1-mediated pathways without broadly affecting related GTPases.
Signal transduction research: W56 is extensively employed in the study of intracellular signaling cascades where Rac1 serves as a critical molecular switch. By selectively inhibiting Rac1, researchers can delineate its involvement in pathways such as the MAPK/ERK and PI3K/AKT axes, which are essential for cell proliferation, survival, and differentiation. The compound enables the precise interrogation of Rac1's upstream and downstream effectors, facilitating the mapping of complex signaling networks and the identification of novel regulatory nodes within these pathways.
Cytoskeletal dynamics studies: The regulation of actin cytoskeleton remodeling is a hallmark function of Rac1, influencing processes such as lamellipodia formation, cell polarity, and directed migration. W56 is utilized to disrupt Rac1-driven actin polymerization, allowing scientists to investigate the molecular underpinnings of cytoskeletal reorganization in various cell types. Through controlled inhibition, it becomes possible to parse out the specific contributions of Rac1 to cell shape changes, adhesion dynamics, and motility, which are critical for understanding tissue development and wound healing at the cellular level.
Cancer cell migration and invasion assays: Aberrant Rac1 signaling has been implicated in the enhanced motility and invasive capacity of malignant cells. W56 serves as a valuable tool in cancer research, where it is used to assess the functional consequences of Rac1 inhibition on tumor cell behavior. By applying this compound in transwell migration, wound healing, or three-dimensional invasion assays, investigators can quantify the impact of Rac1 activity on metastatic potential, thereby advancing the understanding of tumor progression mechanisms and identifying potential targets for future therapeutic intervention.
Neuronal development and plasticity research: In the context of neuroscience, Rac1 is a key regulator of neuronal morphogenesis, dendritic spine formation, and synaptic plasticity. The use of W56 allows for the selective attenuation of Rac1 function in neuronal cultures or brain tissue models, providing insights into the molecular basis of neurite outgrowth, axon guidance, and synapse stability. Such studies are instrumental in elucidating the roles of small GTPases in brain development and in modeling neurodevelopmental disorders at the cellular level.
Cell cycle and proliferation analysis: Rac1 activity is closely linked to cell cycle progression, particularly during the transition from G1 to S phase and in the orchestration of mitotic events. Researchers employ W56 to investigate the consequences of Rac1 inhibition on cell proliferation rates and checkpoint control mechanisms. By integrating this compound into cell cycle assays, it becomes possible to dissect the specific phases and molecular targets affected by Rac1 signaling, contributing to a deeper understanding of cell division regulation and the identification of vulnerabilities in rapidly proliferating cell populations.
2. The spatiotemporal control of signalling and trafficking of the GLP-1R
3. An Open-label, Single-center, Safety and Efficacy Study of Eyelash Polygrowth Factor Serum
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