3,5,3'-Triiodo-D-thyronine is a non-proteinogenic, iodinated thyronine derivative belonging to the thyroid hormone structural class, featuring a diphenyl ether (thyronine) scaffold with three iodine substituents at the 3, 5, and 3' positions and a D-stereochemical configuration as indicated by the name. The molecule contains a phenolic hydroxyl group and a carboxylic acid side chain characteristic of thyronines, giving it both hydrogen-bonding and ionizable functionality while the heavy halogen substitution modulates aromatic electronics and increases chemical mass and polarizability. As a defined, halogenated amino acid-like building block, it is used in chemical biology and analytical method development for studying iodine-substituted thyronine analogues, supporting structure-activity investigations, and serving as a reference substrate in assays that distinguish iodinated thyroid-hormone motifs.
CAT No: CP19402
3,5,3'-Triiodo-D-thyronine is an iodinated thyronine derivative featuring a D-thyronine stereochemical configuration and three iodine substituents on the phenolic rings, making it a structurally defined thyroid hormone analog for chemical and analytical work. Its halogenated, aromatic framework provides strong mass spectrometric detectability and robust chemical identity for method development, while the phenolic groups support established derivatization and assay workflows used to quantify thyroid-related species. In research settings, this compound is handled as a defined small-molecule standard and reference material rather than as a peptide or protein building block.
1. Thyroid Hormone Analytics
3,5,3'-Triiodo-D-thyronine is frequently used to support LC-MS/MS and related analytical method development for thyroid hormone profiling and comparative quantification of iodinated thyronines. Analytical teams rely on its defined iodine substitution pattern and D-configuration to create calibration strategies, retention-time references, and identity-confirmation workflows when separating closely related iodinated species. Because the compound is a discrete small molecule with multiple phenolic functionalities, it is commonly incorporated into analytical panels and validation sets where accurate discrimination between thyroid analogs is required.
2. Isotope-Independent Reference Standard
3,5,3'-Triiodo-D-thyronine serves as a non-isotopic reference standard for instrument qualification, chromatographic system suitability, and routine quality checks in laboratories that measure iodinated thyronines. Researchers use it to verify method consistency across runs, support extraction/cleanup evaluation, and benchmark signal response for phenolic, highly halogenated analytes. This use is particularly common in analytical chemistry and chemical biology groups developing workflows for iodinated aromatic hormone-like compounds, where a stable, well-characterized reference material is needed to interpret results reliably.
3. Chemical Biology Probe Development
3,5,3'-Triiodo-D-thyronine is applied in chemical biology and receptor-binding assay development as a defined thyroid-hormone-like ligand for studying interactions with thyroid hormone binding systems and related pathway components in vitro. Teams developing ligand panels and competition formats use the compound to represent a specific iodination pattern and stereochemical form, enabling structured interpretation of how iodine substitution and stereochemistry influence assay readouts. Its aromatic iodinated scaffold also makes it a practical starting point for designing derivative libraries and for downstream conjugation strategies when a stable, reference ligand is required for probe construction.
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