Z-VDVAD-FMK pairs Z-protection with a VDVAD pentapeptide and an FMK warhead, forming a versatile probe for protease targeting. The sequence tests specificity for alternating hydrophobic and acidic residues. Researchers use it in mechanistic inhibition and enzymatic mapping. Its stable protective group aids synthetic handling and storage.
CAT No: HB00048
Z-VDVAD-FMK is a synthetic peptide-based caspase inhibitor widely utilized in apoptosis research and cell death studies. Structurally, it is a fluoromethyl ketone (FMK) derivative of the VDVAD peptide sequence, designed to irreversibly inhibit caspase-2 activity by covalently modifying the enzyme's active site cysteine. As a cell-permeable and highly selective inhibitor, Z-VDVAD-FMK has become an essential reagent for dissecting the molecular mechanisms of programmed cell death, enabling researchers to probe the specific roles of caspase-2 within complex biochemical pathways. Its utility extends across a range of experimental systems, supporting investigations into cell signaling, neurobiology, immunology, and cancer biology.
Apoptosis pathway analysis: In the context of cell death research, Z-VDVAD-FMK is a valuable tool for selectively blocking caspase-2 activity, allowing scientists to delineate the contribution of this protease to apoptotic signaling cascades. By inhibiting caspase-2, researchers can distinguish its unique role from other caspases, facilitating detailed analysis of mitochondrial dysfunction, cytochrome c release, and downstream effector activation. This approach is instrumental in mapping cell fate decisions and understanding the molecular checkpoints that govern apoptosis in various cell types.
Mechanistic studies of caspase specificity: The compound's peptide-based sequence confers high selectivity, making it ideal for studies aimed at characterizing substrate recognition and cleavage specificity among caspase family members. By employing Z-VDVAD-FMK in in vitro assays or cell-based models, investigators can explore the biochemical determinants of caspase-2 substrate preference, elucidate cross-talk with other proteolytic enzymes, and validate the selectivity of novel caspase-targeted probes. Such studies provide critical insight into the structural and functional diversity of caspase-mediated proteolysis.
Neurodegeneration and stress response modeling: Research into neurodegenerative processes frequently leverages caspase inhibitors to dissect the molecular underpinnings of neuronal loss and stress-induced cell death. Z-VDVAD-FMK is routinely applied in neuronal culture systems to block caspase-2-dependent apoptosis, enabling the investigation of oxidative stress responses, DNA damage repair mechanisms, and synaptic integrity. Its use facilitates the identification of neuroprotective pathways and enhances understanding of the cellular events leading to neurodegeneration.
Cancer cell biology and drug screening: In oncology research, the selective inhibition of caspase-2 using FMK-based peptides supports the evaluation of cell survival, proliferation, and resistance mechanisms in tumor cell lines. By modulating caspase activity, researchers can assess the impact of genetic or pharmacological interventions on apoptosis sensitivity, delineate the interplay between cell cycle regulation and programmed cell death, and identify potential biomarkers for therapeutic response. Z-VDVAD-FMK is frequently incorporated into high-content screening platforms to validate the efficacy and specificity of candidate anticancer compounds.
Immunological signaling investigations: The role of caspases in immune cell function, including lymphocyte activation and cytokine processing, is an area of active research. Application of this peptide inhibitor in immune cell assays allows for the targeted inhibition of caspase-2, facilitating the study of its involvement in T cell receptor signaling, inflammasome activation, and immune tolerance. Such investigations contribute to a deeper understanding of immune regulation and the molecular basis of inflammatory disorders, supporting the development of targeted immunomodulatory strategies.
2. An Open-label, Single-center, Safety and Efficacy Study of Eyelash Polygrowth Factor Serum
3. Peptides as Active Ingredients: A Challenge for Cosmeceutical Industry
5. Implications of ligand-receptor binding kinetics on GLP-1R signalling
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More