Bim BH3 represents a helix-forming peptide segment commonly examined for protein-protein interaction modeling. Its hydrophobic-core residues promote α-helical formation in membrane-mimetic systems. Researchers study its folding kinetics and binding specificity to understand regulatory networks. Uses include structural biology, motif characterization, and peptide mimic design.
CAT No: R2626
CAS No:505070-06-4
Synonyms/Alias:Bim BH3;EX-A9193;HY-P1527;CS-0044788;505070-06-4;
Bim BH3 is a synthetic peptide corresponding to the BH3 domain of the Bcl-2-interacting mediator of cell death (Bim) protein, a pro-apoptotic member of the Bcl-2 family. As a highly conserved alpha-helical motif, the BH3 domain plays a pivotal role in regulating mitochondrial apoptosis by mediating protein-protein interactions within the Bcl-2 family. The biochemical significance of Bim BH3 lies in its ability to selectively bind and neutralize anti-apoptotic Bcl-2 proteins, thereby promoting the activation of downstream effectors such as Bax and Bak. This peptide is widely utilized in molecular and cellular studies to dissect apoptotic pathways, understand protein interactions critical for cell fate decisions, and develop novel strategies for apoptosis modulation in various biological models.
Apoptosis pathway analysis: Bim BH3 peptide is extensively employed in mechanistic studies of programmed cell death, particularly in delineating the intrinsic mitochondrial apoptosis pathway. By mimicking the functional BH3 domain, it serves as a molecular tool to trigger or sensitize cells to apoptosis in vitro, enabling researchers to investigate the sequence of molecular events following mitochondrial outer membrane permeabilization. The use of this peptide facilitates detailed exploration of caspase activation, cytochrome c release, and the interplay between pro- and anti-apoptotic Bcl-2 family members under controlled experimental conditions.
Protein-protein interaction assays: Researchers utilize Bim BH3 in binding studies to characterize interactions between BH3-only proteins and their anti-apoptotic counterparts, such as Bcl-2, Bcl-xL, and Mcl-1. Through techniques like fluorescence polarization, surface plasmon resonance, and co-immunoprecipitation, the peptide enables quantitative and qualitative assessment of binding affinities, competitive inhibition, and specificity. These insights are instrumental for mapping the structural determinants of BH3-mediated interactions and for screening potential modulators of Bcl-2 family function.
Drug discovery and inhibitor screening: The synthetic Bim BH3 domain is a valuable reagent for high-throughput screening platforms aimed at identifying small molecules or peptides that modulate Bcl-2 family protein interactions. By serving as a reference ligand or competitive substrate in assay systems, it supports the evaluation of candidate compounds that disrupt anti-apoptotic protein binding, thereby accelerating the preclinical development of apoptosis-inducing agents for research applications. Such studies contribute foundational knowledge for the rational design of targeted modulators of cell death pathways.
Structural biology and conformational studies: Bim BH3 peptide is frequently incorporated into structural investigations, including X-ray crystallography and nuclear magnetic resonance spectroscopy, to resolve the three-dimensional architecture of Bcl-2 family complexes. By providing a defined interaction motif, it enables the elucidation of binding interfaces, conformational changes, and allosteric effects upon complex formation. These structural insights are critical for understanding the molecular basis of apoptosis regulation and for guiding the design of novel peptide mimetics or inhibitors.
Cell-based functional assays: In cellular models, Bim BH3 is introduced to probe the functional consequences of BH3 domain-mediated interactions in live cells. Researchers employ the peptide to induce apoptosis, study mitochondrial membrane dynamics, and assess the contribution of specific Bcl-2 family members to cell survival. Such functional assays are essential for validating mechanistic hypotheses generated from in vitro studies and for exploring the context-dependent roles of apoptotic regulators in diverse biological systems.
1. Immune-awakening Saccharomyces-inspired nanocarrier for oral target delivery to lymph and tumors
2. Low bone turnover and low BMD in Down syndrome: effect of intermittent PTH treatment
3. Cationic cell-penetrating peptides are potent furin inhibitors
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More