Fmoc-Ala-OH-13C3 is an Fmoc-protected alanine derivative bearing a carboxylic acid functional group and an amino group masked with the fluorenylmethoxycarbonyl (Fmoc) protecting group, with three carbon atoms labeled as 13C. The molecule features the alanine side chain (a methyl group) attached to the alpha carbon, and its stereochemistry corresponds to the alanine framework as defined by the supplied structure while retaining the free carboxyl group for subsequent coupling chemistry. In peptide synthesis workflows and amino acid labeling studies, the 13C3 isotopic enrichment supports mass spectrometric tracking or NMR-based quantitation, while the Fmoc group enables stepwise assembly of peptide chains by controlling the reactivity of the amino functionality.
CAT No: HB00064
Fmoc-Ala-OH-13C3 is an Fmoc-protected alanine building block bearing a 13C3 stable isotope label, supplied in a form commonly used for downstream peptide assembly and quantitative analytical workflows. The labeled methyl carbon(s) provide a defined mass shift for LC-MS-based detection and allow researchers to track or normalize alanine-containing peptide segments with isotopic specificity. As an amino acid derivative designed for peptide synthesis chemistry, it features the Fmoc group for controlled coupling chemistry while retaining the alanine side chain for incorporation into defined sequences.
1. Isotope-Labeled Peptide Synthesis
Fmoc-Ala-OH-13C3 is used to prepare isotopically defined peptides and peptide standards where alanine residues must carry a known 13C mass signature. Peptide synthesis groups in chemical biology, proteomics method development, and analytical chemistry routinely select this labeled Fmoc amino acid to generate reference peptides for calibration, identification, and relative quantification workflows that rely on the mass difference between labeled and unlabeled species. The Fmoc protection supports standard peptide assembly strategies, enabling incorporation into short peptides, internal standards, or sequence-specific segments used to validate digestion and measurement performance.
2. Quantitative LC-MS Internal Standards
Fmoc-Ala-OH-13C3 is frequently incorporated into peptide standards used as internal references for LC-MS quantification, especially when alanine-containing fragments are monitored. Analytical laboratories use isotopically labeled peptide analogs to reduce run-to-run variability by anchoring signal response to a mass-resolved labeled counterpart. The 13C3 enrichment pattern provides a predictable isotopic signature that can be resolved in MS workflows, supporting more robust normalization for targeted assays, method qualification, and comparative studies where alanine-derived fragments contribute to the measured signal.
3. Proteomics and Targeted Assay Development
Fmoc-Ala-OH-13C3 supports targeted proteomics and proteome workflow development by enabling the synthesis of labeled peptide reagents that mimic specific tryptic or enzymatic digestion outcomes containing alanine. Proteomics researchers use these labeled peptides to evaluate instrument settings, optimize acquisition parameters, and verify selectivity in targeted MS experiments such as multiple reaction monitoring-style approaches. By building isotopic labels directly into peptide sequences during synthesis, teams can generate consistent standards that align with the chemistry and fragmentation behavior of the analyte peptides used in downstream protein quantification pipelines.
4. Stable Isotope Tracing Controls
Fmoc-Ala-OH-13C3 is also used to create labeled peptide controls for experiments where isotopic composition must be tracked through sample processing and measurement. In metabolic research and chemical biology workflows that involve peptide-level readouts, researchers may use labeled alanine-containing peptide standards to monitor recovery, digestion efficiency, and analytical consistency across conditions. The defined 13C labeling enables discrimination from unlabeled background and supports interpretation of isotope-dependent signals when alanine-containing peptide species serve as measurable proxies in the experimental design.
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