Protein SSX4 (51-70)

Protein SSX4 (51-70) is a synthetic peptide fragment derived from the SSX4 protein, a member of the synovial sarcoma X breakpoint (SSX) family. This peptide encompasses amino acid residues 51 through 70 of the parent protein, representing a region that may be functionally significant in protein-protein interactions and molecular recognition processes. As a chemically defined peptide, it provides researchers with a precise tool for dissecting the structural and functional dynamics of the SSX4 protein, which is implicated in chromatin remodeling and transcriptional regulation. The availability of this sequence-specific fragment enables targeted experimental approaches in molecular biology, biochemistry, and proteomics, supporting a wide range of peptide-based investigations.

Epitope mapping: The SSX4 (51-70) peptide is frequently utilized in epitope mapping studies to identify immunologically relevant regions within the SSX4 protein. By employing this defined segment in immunoassays or binding studies, researchers can determine antibody recognition sites and characterize immune responses specific to this region. Such mapping is essential for understanding the antigenic landscape of SSX family proteins, contributing to the development of research antibodies and advancing knowledge of protein immunogenicity.

Protein interaction analysis: In the context of protein-protein interaction studies, this peptide serves as a valuable probe for investigating the binding specificity of SSX4 with its molecular partners. By incorporating the 51-70 fragment into binding assays or pull-down experiments, scientists can elucidate the role of this sequence in mediating interactions with chromatin-associated factors or transcriptional regulators. These insights are instrumental in unraveling the molecular mechanisms underlying SSX4 function and its involvement in cellular regulatory networks.

Peptide functional studies: The SSX4 (51-70) fragment is also employed in functional assays aimed at dissecting the biological activity of discrete protein regions. By introducing the peptide into cellular or in vitro systems, researchers can assess its influence on processes such as transcriptional repression, chromatin modification, or signal transduction pathways. This targeted approach allows for the delineation of sequence-specific effects, facilitating a deeper understanding of structure-function relationships within the SSX4 protein.

Antibody generation: As a chemically synthesized, sequence-defined antigen, the SSX4 (51-70) peptide is well-suited for use in the production of polyclonal or monoclonal antibodies. Its application as an immunogen enables the generation of antibodies with specificity for the corresponding region of the native protein, supporting downstream research applications including immunoblotting, immunoprecipitation, and immunofluorescence. The use of such region-specific antibodies enhances the precision of protein detection and quantification in complex biological samples.

Mass spectrometry standards: In proteomics workflows, the SSX4 (51-70) peptide can be employed as a reference standard for mass spectrometry-based analyses. Its defined sequence and physicochemical properties facilitate the calibration of analytical instruments and the validation of peptide identification protocols. Incorporating this peptide into quantitative assays or spectral libraries supports robust detection and accurate quantification of SSX4-derived peptides in biological samples, thereby advancing proteomic profiling and biomarker discovery efforts.

1. Adipose tissue is a key organ for the beneficial effects of GLP-2 metabolic function

2. Urinary Metabolites Associated with Blood Pressure on a Low-or High-Sodium Die

3. Therapeutic potential of an intestinotrophic hormone, glucagon-like peptide 2, for treatment of type 2 short bowel syndrome rats with intestinal bacterial and fungal dysbiosis

4. An Isolated TCR αβ Restricted by HLA-A* 02: 01/CT37 Peptide Redirecting CD8+ T Cells to Kill and Secrete IFN-γ in Response to Lung Adenocarcinoma Cell Lines

5. Relatedness of antibodies to peptides containing homocitrulline or citrulline in patients with rheumatoid arthritis