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This is a a sensitive fluorogenic substrate for elastase and trypsin, Abs/Em=501/527 nm. The colorless and nonfluorescent (Z-Ala-Arg)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. After cleavage, the Abs(max) is at 497 nm and Em(max): is at 527 nm.
Peptide Library Construction and Screening
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M.W/Mr. | 1053.1 |
Sequence | One Letter Code: (Z-AR)2Rh110•HCl Three Letter Code: (Z-Ala-Arg)2Rh110•2HCl |
Long-term Storage Conditions | DMSO |
References | 1. Devi S, et al. (1995). Biologic activities of the beta-chemokine TCA3 on neutrophils and macrophages. J Immunol 154, 5376-83, 2. Leytus SP, et al. (1983). New class of sensitive and selective fluorogenic substrates for serine proteinases. Amino acid and dipeptide derivatives of rhodamine. Biochem J 215, 253-60. |
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