lymphocyte antigen 6 complex locus K (114-133)

Lymphocyte antigen 6 complex locus K (114-133) is a synthetic peptide fragment derived from the Ly6K protein, a member of the Ly6/uPAR superfamily known for its role in immune cell signaling and cell surface interactions. This peptide encompasses amino acids 114 to 133 of the parent protein, representing a region that may be relevant for antigenicity, protein-protein interactions, and functional mapping studies. As a research-grade peptide, it offers valuable opportunities for dissecting the molecular mechanisms underlying Ly6K-mediated pathways, facilitating the investigation of immune modulation, cell recognition processes, and potential post-translational modifications. Its defined sequence and biochemical characteristics make it a versatile tool for a range of experimental applications in immunology, molecular biology, and protein chemistry.

Epitope mapping: Researchers utilize the 114-133 peptide fragment to identify and characterize epitopes recognized by antibodies or immune receptors targeting Ly6K. By serving as a defined antigenic sequence, the peptide enables precise determination of antibody binding sites, supporting the development of monoclonal antibodies and facilitating studies on immune recognition. Such mapping is crucial for understanding the specificity of immune responses and for designing reagents that selectively detect or modulate Ly6K-related pathways in cellular assays.

Antibody production: The peptide is frequently employed as an immunogen for generating polyclonal or monoclonal antibodies specific to the Ly6K protein. Its use as a synthetic antigen allows for the production of highly specific antibodies that target the 114-133 region, which can be leveraged in downstream applications such as Western blotting, immunohistochemistry, or flow cytometry. The ability to raise antibodies against a defined peptide sequence enhances the specificity and reproducibility of immunodetection techniques, supporting both basic research and assay development.

Protein interaction studies: The 114-133 fragment serves as a valuable probe for investigating protein-protein interactions involving Ly6K. By providing a discrete segment of the protein, the peptide can be used in binding assays, pull-down experiments, or surface plasmon resonance studies to elucidate interaction partners or binding affinities. Such analyses contribute to the understanding of Ly6K's functional roles in cellular signaling networks and may reveal novel molecular interactions relevant to immune regulation or cell adhesion.

T cell response assays: Synthetic peptides corresponding to defined regions of immune-related proteins, such as Ly6K (114-133), are instrumental in assessing T cell responses in vitro. The peptide can be used to stimulate lymphocytes in proliferation assays, cytokine release assays, or ELISPOT analyses, enabling the evaluation of antigen-specific cellular immunity. These assays are essential for dissecting the immunogenic potential of Ly6K-derived sequences and for investigating mechanisms of immune recognition and tolerance.

Peptide functionalization and assay development: The defined biochemical properties of the 114-133 sequence make it suitable for conjugation to carrier proteins, labeling with fluorescent or affinity tags, or immobilization on assay platforms. Such functionalization expands its utility in the development of peptide-based detection systems, biosensors, and high-throughput screening assays. By providing a consistent and reproducible reagent, the peptide supports the advancement of analytical techniques aimed at quantifying Ly6K expression, monitoring immune responses, or screening for modulators of Ly6K-associated pathways.

1. Genetic, cellular, and structural characterization of the membrane potential-dependent cell-penetrating peptide translocation pore

2. Myotropic activity of allatostatins in tenebrionid beetles

3. C-Peptide replacement therapy and sensory nerve function in type 1 diabetic neuropathy

4. Olfactory receptor based piezoelectric biosensors for detection of alcohols related to food safety applications

5. Multifunctional peptide-oligonucleotide conjugate promoted sensitive electrochemical biosensing of cardiac troponin I