Neuropeptide FF (NPFF), an octapeptide belonging to the RF-amide family of peptides, interacts with two distinct G-protein-coupled receptors, NPFF(1) and NPFF(2) and has wide variety of physiological functions in the brain including central cardiovascular and neuroendocrine regulation.
CAT No: R1542
CAS No:99566-27-5
Synonyms/Alias:Neuropeptide FF;99566-27-5;NPFF;MFCD00076704;CHEMBL429731;phe-leu-phe-gln-pro-gln-arg-phe-amide;PrRP31;NPSF;Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe amide;[125I]-neuropeptide FF;Neuropeptide ff(cattle)(9ci);[125I]NPFF;GTPL3797;[125I]-NPFF;BDBM86139;HY-P1248;BDBM50037557;AKOS024457451;NCGC00167257-01;DA-66042;MS-31938;CAS_99566-27-5;CAS_235433-36-0;CS-0029121;Neuropeptide FF Morphine Modulating Neuropeptide F-8-F-NH2;Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe amide, >=95% (HPLC);(2S)-2-[[(2S)-1-[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]-3-phenylpropanoyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-N-[(2S)-1-[[(2S)-1-amino-1-oxo-3-phenylpropan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]pentanediamide;2-{[1-(2-{2-[2-(2-Amino-3-phenyl-propionylamino)-4-methyl-pentanoylamino]-3-phenyl-propionylamino}-4-carbamoyl-butyryl)-pyrrolidine-2-carbonyl]-amino}-pentanedioic acid 5-amide 1-{[1-(1-carbamoyl-2-phenyl-ethylcarbamoyl)-4-guanidino-butyl]-amide};
Neuropeptide FF is a biologically active peptide that plays a significant role in the modulation of pain, neuroendocrine regulation, and cardiovascular function. As an endogenous member of the RFamide peptide family, it is characterized by its conserved C-terminal Arg-Phe-NH2 motif, which is critical for receptor binding and activity. Neuropeptide FF is widely recognized for its involvement in the central and peripheral nervous systems, where it acts as a neuromodulator and interacts with specific G protein-coupled receptors. Its ability to influence various physiological pathways has positioned it as a valuable tool in neuropharmacological research and functional peptide studies.
Pain modulation research: Neuropeptide FF has been extensively utilized in studies investigating the mechanisms of pain perception and analgesia. Researchers employ this peptide to dissect its dual role in both enhancing and attenuating opioid-induced analgesia, providing insights into the complex interplay between endogenous opioid systems and RFamide peptides. Experimental applications include the administration of the peptide in in vitro and in vivo models to examine its effects on nociceptive signaling and its potential to modulate opioid tolerance and hyperalgesia. These studies contribute to a deeper understanding of peptide-mediated pain pathways and the development of novel analgesic strategies.
Neuroendocrine signaling studies: The peptide serves as a model system for exploring neuroendocrine regulation, particularly in the hypothalamic-pituitary axis. Investigators utilize Neuropeptide FF to probe its influence on hormone secretion, stress responses, and metabolic homeostasis. By applying it to neuronal cultures or animal models, researchers can elucidate its effects on the release of key neurohormones such as vasopressin and oxytocin, thereby advancing knowledge of neuropeptide-driven endocrine control mechanisms.
Cardiovascular function analysis: Neuropeptide FF is employed in cardiovascular research to investigate its modulatory effects on blood pressure and heart rate. Experimental protocols often involve administering the peptide to isolated tissue preparations or whole-animal systems to observe its impact on sympathetic and parasympathetic activity. Such studies have revealed its capacity to influence baroreflex sensitivity and vascular tone, making it a useful probe for dissecting the neuropeptidergic regulation of cardiovascular dynamics.
Peptide-receptor interaction assays: The compound is frequently used in receptor binding and signaling assays to characterize the pharmacology of the NPFF1 and NPFF2 receptors. Through radioligand binding studies, functional assays, and receptor activation profiling, researchers can delineate the specificity, affinity, and downstream signaling pathways associated with RFamide receptors. These investigations are fundamental for understanding receptor-ligand interactions and for the development of selective agonists or antagonists targeting this peptide system.
Peptide structure-function analysis: Neuropeptide FF is also valuable in structure-activity relationship (SAR) studies aimed at identifying the critical residues and structural motifs responsible for its biological activity. By synthesizing and testing analogs or truncated forms, scientists can map the functional domains essential for receptor binding and signal transduction. These SAR investigations inform the rational design of novel bioactive peptides and contribute to advancing peptide-based research tools and therapeutics in the neuropeptide field.
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